乳及乳制品中分离肠球菌毒力及耐药基因的分子检测

Alaa Abouzaid, M. A. Abd Al-azeem, Hams M. A. Mohamed
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引用次数: 0

摘要

肠球菌是最严重的病原体之一,可能对动物和人类构成风险,也是编码抗生素耐药性的基因库,可以转移到其他微生物。在过去的十年里,肠球菌成为最常见的人类腹部或骨盆感染、败血症和肠球菌脑膜炎的病因。因此,本研究旨在重点研究这些微生物的毒力,并揭示肠球菌耐药性和生物膜形成之间的联系。从埃及基纳省的不同市场共采集了120份生牛奶和奶酪样本。这些样品被怀疑用于微生物分析,如培养、生化方法、VITEK2系统和PCR技术,用于检测抗性和毒力基因。常规鉴定表明,30个分离株被怀疑为肠球菌。VITEK 2系统确认这些分离株为肠球菌,并将它们分为15个分离株为粪肠球菌(E.faecalis),10个分离株是粪肠球菌(E.faecaium),而种类最少的是5个分离株,为鸡肠球菌(E.galinarum)。此外,我们的分离株表现出形成中等和弱生物膜的能力,分别为66.67%和16.67%。药敏结果显示对青霉素、红霉素、阿莫西林、呋喃妥因和头孢噻肟具有较高的耐药性。我们的研究加剧了人们对肠球菌中生物膜形成与特定类型的抗微生物耐药性之间的重要关系的担忧。如头孢噻肟、青霉素、氨苄青霉素和红霉素。筛选出10株分离株,用聚合酶链式反应检测其抗微生物耐药性和生物膜基因。本研究获得的分离株具有不同的抗微生物基因,包括erm B(80%)、bla Z(100%)和van A(30%),生物膜形成基因包括hyl和ef3 314(100%),esp(90%)。最后,我们包括,通过采取严格的卫生措施,遏制牛奶及其产品中肠球菌的污染是必要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular detection of virulence and resistance genes of Enterococcus species isolated from milk and milk products
Enterococcus species are one of the most serious pathogens that could pose risk to animal and human also a reservoir of genes encoding antibiotic resistance which can be transferred to other microorganisms. In the last decade, Enterococci become the most frequently reported cause of an abdominal or pelvic infection, septicemia, and enterococcal meningitis in humans. So, this study aimed to focus alight on virulence of these microbes and to show the linkage between antimicrobial resistance and biofilm formation in Enterococcus spp . A total of 120 samples of raw milk and cheese were collected from different markets in Qena province, Egypt. These samples were suspected for microbial analysis as culture, biochemical method, VITEK2 system and PCR technique for detection of resistance and virulence genes. The conventional identification showed that 30 isolates were suspected to be Enterococcus spp., VITEK 2 system confirmed the isolates as Enterococcus and classified them into 15 isolates as Enterococcus faecalis ( E. faecalis ),10 isolates as Enterococcus faecium ( E. faecium ) whereas the least species were 5 isolates were Enterococcus gallinarum ( E. gallinarum ). also, our isolates showed their ability to form moderate and weak of biofilm with percentage%66.67% and 16.67% respectively. Also, the results of antimicrobial susceptibility showed high resistance against penicillin, Erythromycin, Amoxicillin, Nitrofurantoin and Cefotaxime. Our study is fueling the concern on a significant relationship between biofilm formation and specific types of antimicrobial resistance in Enterococcus spp. as Cefotaxime, Penicillin, Ampicillin and Erythromycin. Ten isolates were selected and tested by PCR for detection antimicrobial resistance and biofilm genes. The isolates obtained in this study harbored different antimicrobial resistance genes included erm B (80%), bla Z (100%) and van A (30%) and biofilm formation genes included hyl and ef3 314 (100%), esp (90%). Finally, we included, the deterrence of the contamination by Enterococcus spp. in milk and its products becomes necessary by applying strict hygienic measure.
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