{"title":"局部应用冻干和粉末状人羊膜促进糖尿病溃疡愈合","authors":"Anna Rodella , Michela Pozzobon , Matteo Rigon , Cinzia Franchin , Giorgio Arrigoni , Manuela Simonato , Emiliano Ghinelli , Luca Vedovelli","doi":"10.1016/j.wndm.2019.100171","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Amniotic membrane has been widely used in the treatment of several acute and chronic diseases, and surgical reconstructions. Here we report the successful application of a commercial lyophilized, powdered, amniotic membrane (AMX) to a patient with a treatment-refractory diabetic ulcer, with further investigations on AMX angiogenic and anti-inflammatory properties.</p></div><div><h3>Objective</h3><p>To investigate the topical, continuous, treatment with AMX on a chronic diabetic foot ulcer, along with its angiogenic <em>in vitro</em> properties and proteomic profile.</p></div><div><h3>Methods</h3><p>A 77-year old woman was treated with AMX 2–3 drops 4–6 times a day for 2 months. Amniotic fluid stem cells were tested for proliferation and angiogenesis potential with or without AMX. ELISA quantification was conducted on NGF, TGF-alpha, NT-3, and IL1-ra. Proteomic analysis was also performed on AMX.</p></div><div><h3>Results</h3><p>At 2-month follow-up, the ulcer was reduced by 60% and it remained steady after 3-months. At 4-month follow-up, the lesion showed signs of re-epithelization. Cells, with or without AMX, were prone to form endothelial tubules with similar characteristics. Proteomic analysis confirmed that AMX retains proteins involved in anti-inflammatory and angiogenesis pathways.</p></div><div><h3>Conclusions</h3><p>AMX ameliorated pain and extent of a chronic diabetic ulcer. The effect could be ascribed to angiogenesis and epithelium stimulation that was confirmed in <em>in vitro</em>. Multiple wound healing-involved proteins were detected but further studies are needed to assess their specific role.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"27 1","pages":"Article 100171"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100171","citationCount":"2","resultStr":"{\"title\":\"Topical application of lyophilized and powdered human amniotic membrane promotes diabetic ulcer healing\",\"authors\":\"Anna Rodella , Michela Pozzobon , Matteo Rigon , Cinzia Franchin , Giorgio Arrigoni , Manuela Simonato , Emiliano Ghinelli , Luca Vedovelli\",\"doi\":\"10.1016/j.wndm.2019.100171\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Amniotic membrane has been widely used in the treatment of several acute and chronic diseases, and surgical reconstructions. Here we report the successful application of a commercial lyophilized, powdered, amniotic membrane (AMX) to a patient with a treatment-refractory diabetic ulcer, with further investigations on AMX angiogenic and anti-inflammatory properties.</p></div><div><h3>Objective</h3><p>To investigate the topical, continuous, treatment with AMX on a chronic diabetic foot ulcer, along with its angiogenic <em>in vitro</em> properties and proteomic profile.</p></div><div><h3>Methods</h3><p>A 77-year old woman was treated with AMX 2–3 drops 4–6 times a day for 2 months. Amniotic fluid stem cells were tested for proliferation and angiogenesis potential with or without AMX. ELISA quantification was conducted on NGF, TGF-alpha, NT-3, and IL1-ra. Proteomic analysis was also performed on AMX.</p></div><div><h3>Results</h3><p>At 2-month follow-up, the ulcer was reduced by 60% and it remained steady after 3-months. At 4-month follow-up, the lesion showed signs of re-epithelization. Cells, with or without AMX, were prone to form endothelial tubules with similar characteristics. Proteomic analysis confirmed that AMX retains proteins involved in anti-inflammatory and angiogenesis pathways.</p></div><div><h3>Conclusions</h3><p>AMX ameliorated pain and extent of a chronic diabetic ulcer. The effect could be ascribed to angiogenesis and epithelium stimulation that was confirmed in <em>in vitro</em>. Multiple wound healing-involved proteins were detected but further studies are needed to assess their specific role.</p></div>\",\"PeriodicalId\":38278,\"journal\":{\"name\":\"Wound Medicine\",\"volume\":\"27 1\",\"pages\":\"Article 100171\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100171\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Wound Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213909518300272\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Wound Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213909518300272","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Topical application of lyophilized and powdered human amniotic membrane promotes diabetic ulcer healing
Background
Amniotic membrane has been widely used in the treatment of several acute and chronic diseases, and surgical reconstructions. Here we report the successful application of a commercial lyophilized, powdered, amniotic membrane (AMX) to a patient with a treatment-refractory diabetic ulcer, with further investigations on AMX angiogenic and anti-inflammatory properties.
Objective
To investigate the topical, continuous, treatment with AMX on a chronic diabetic foot ulcer, along with its angiogenic in vitro properties and proteomic profile.
Methods
A 77-year old woman was treated with AMX 2–3 drops 4–6 times a day for 2 months. Amniotic fluid stem cells were tested for proliferation and angiogenesis potential with or without AMX. ELISA quantification was conducted on NGF, TGF-alpha, NT-3, and IL1-ra. Proteomic analysis was also performed on AMX.
Results
At 2-month follow-up, the ulcer was reduced by 60% and it remained steady after 3-months. At 4-month follow-up, the lesion showed signs of re-epithelization. Cells, with or without AMX, were prone to form endothelial tubules with similar characteristics. Proteomic analysis confirmed that AMX retains proteins involved in anti-inflammatory and angiogenesis pathways.
Conclusions
AMX ameliorated pain and extent of a chronic diabetic ulcer. The effect could be ascribed to angiogenesis and epithelium stimulation that was confirmed in in vitro. Multiple wound healing-involved proteins were detected but further studies are needed to assess their specific role.
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