纳米金舌下免疫治疗哮喘小鼠模型

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Omid Javanmardghooghan, Fatemeh Azmoudeh, Mohammadamin Sadeghdoust, Farnaz Aligolighasemabadi, M. Khakzad
{"title":"纳米金舌下免疫治疗哮喘小鼠模型","authors":"Omid Javanmardghooghan, Fatemeh Azmoudeh, Mohammadamin Sadeghdoust, Farnaz Aligolighasemabadi, M. Khakzad","doi":"10.22038/NMJ.2021.08.005","DOIUrl":null,"url":null,"abstract":"Objective(s): It has been shown that Nanogold particles have anti-inflammatory effects in different Rheumatologic, neurologic and gastrointestinal disease. They inhibit the synthesis of pro-inflammatory cytokines and also infiltration of inflammatory cells. Sublingual immunotherapy is a well-known effective, safe and clinically effective method way of immune response regulation which results in long-lasting symptoms reduction. This research was designed to find the immunological effects of sublingual immunotherapy using Nanogold in mice model of asthma. Materials and Methods: Twenty BALB/c mice were divided into four groups including one group of non-sensitized mice and three groups of asthmatic mice which were treated sublingually with PBS, Nanogold and Beclomethasone. IL-4 and IFN-γ levels were measured in serum and spleen cells supernatant using ELISA. BAL fluid inflammatory cells differential counting and lungs histological analysis were also done.Results: The results revealed that there was significant increase in level of IFN-γ and decrease in level of IL-4 in serum and spleen cells supernatant of Nanogold treated group (p <0.05). These findings indicates the shift of Th2/Th1 balance towards Th1 cells which is protective against asthma. In addition, histological and BAL fluid analysis demonstrated the reduction of cells and eosinophilic infiltration. Conclusion: Based on our results, sublingual immunotherapy by Nanogold has significant anti-inflammatory roll in asthmatic mice. Thus, Nanogold is a potentially valuable agent for controlling the underlying inflammation in asthma. However, further investigations is recommended to find more details about its effects.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sublingual immunotherapy by Nanogold in mice model of asthma\",\"authors\":\"Omid Javanmardghooghan, Fatemeh Azmoudeh, Mohammadamin Sadeghdoust, Farnaz Aligolighasemabadi, M. Khakzad\",\"doi\":\"10.22038/NMJ.2021.08.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective(s): It has been shown that Nanogold particles have anti-inflammatory effects in different Rheumatologic, neurologic and gastrointestinal disease. They inhibit the synthesis of pro-inflammatory cytokines and also infiltration of inflammatory cells. Sublingual immunotherapy is a well-known effective, safe and clinically effective method way of immune response regulation which results in long-lasting symptoms reduction. This research was designed to find the immunological effects of sublingual immunotherapy using Nanogold in mice model of asthma. Materials and Methods: Twenty BALB/c mice were divided into four groups including one group of non-sensitized mice and three groups of asthmatic mice which were treated sublingually with PBS, Nanogold and Beclomethasone. IL-4 and IFN-γ levels were measured in serum and spleen cells supernatant using ELISA. BAL fluid inflammatory cells differential counting and lungs histological analysis were also done.Results: The results revealed that there was significant increase in level of IFN-γ and decrease in level of IL-4 in serum and spleen cells supernatant of Nanogold treated group (p <0.05). These findings indicates the shift of Th2/Th1 balance towards Th1 cells which is protective against asthma. In addition, histological and BAL fluid analysis demonstrated the reduction of cells and eosinophilic infiltration. Conclusion: Based on our results, sublingual immunotherapy by Nanogold has significant anti-inflammatory roll in asthmatic mice. Thus, Nanogold is a potentially valuable agent for controlling the underlying inflammation in asthma. However, further investigations is recommended to find more details about its effects.\",\"PeriodicalId\":18933,\"journal\":{\"name\":\"Nanomedicine Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2021-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanomedicine Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22038/NMJ.2021.08.005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"NANOSCIENCE & NANOTECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/NMJ.2021.08.005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目的:研究表明纳米金颗粒对不同的风湿病、神经系统疾病和胃肠道疾病具有抗炎作用。它们抑制促炎细胞因子的合成和炎症细胞的浸润。舌下免疫治疗是一种众所周知的有效、安全、临床有效的免疫反应调节方法,可持久减轻症状。本研究旨在观察纳米金舌下免疫治疗对哮喘小鼠模型的免疫效果。材料与方法:将20只BALB/c小鼠分为4组,其中1组为非致敏小鼠,3组为哮喘小鼠,分别用PBS、纳米金和倍氯米松舌下治疗。ELISA法检测血清和脾细胞上清中IL-4和IFN-γ水平。BAL液炎性细胞鉴别计数及肺组织分析。结果:纳米金处理组大鼠血清和脾细胞上清液中IFN-γ水平显著升高,IL-4水平显著降低(p <0.05)。这些发现表明Th2/Th1平衡向Th1细胞转移,这对哮喘有保护作用。此外,组织学和BAL液分析显示细胞和嗜酸性粒细胞浸润减少。结论:纳米金舌下免疫治疗对哮喘小鼠有明显的抗炎作用。因此,纳米金是一种潜在的有价值的药物,可以控制哮喘的潜在炎症。然而,建议进行进一步调查,以找到有关其影响的更多细节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sublingual immunotherapy by Nanogold in mice model of asthma
Objective(s): It has been shown that Nanogold particles have anti-inflammatory effects in different Rheumatologic, neurologic and gastrointestinal disease. They inhibit the synthesis of pro-inflammatory cytokines and also infiltration of inflammatory cells. Sublingual immunotherapy is a well-known effective, safe and clinically effective method way of immune response regulation which results in long-lasting symptoms reduction. This research was designed to find the immunological effects of sublingual immunotherapy using Nanogold in mice model of asthma. Materials and Methods: Twenty BALB/c mice were divided into four groups including one group of non-sensitized mice and three groups of asthmatic mice which were treated sublingually with PBS, Nanogold and Beclomethasone. IL-4 and IFN-γ levels were measured in serum and spleen cells supernatant using ELISA. BAL fluid inflammatory cells differential counting and lungs histological analysis were also done.Results: The results revealed that there was significant increase in level of IFN-γ and decrease in level of IL-4 in serum and spleen cells supernatant of Nanogold treated group (p <0.05). These findings indicates the shift of Th2/Th1 balance towards Th1 cells which is protective against asthma. In addition, histological and BAL fluid analysis demonstrated the reduction of cells and eosinophilic infiltration. Conclusion: Based on our results, sublingual immunotherapy by Nanogold has significant anti-inflammatory roll in asthmatic mice. Thus, Nanogold is a potentially valuable agent for controlling the underlying inflammation in asthma. However, further investigations is recommended to find more details about its effects.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信