仿生铁磁性CoFe2O4纳米颗粒:潜在的药物和医学应用

IF 6.1 3区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
M. Ansari, R. Govindasamy, M. Y. Begum, Mohammed Ghazwani, A. Alqahtani, M. Alomary, Yahya F. Jamous, S. Alyahya, S. Asiri, F. Khan, M. Almessiere, A. Baykal
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引用次数: 1

摘要

摘要本工作的主要目标是开发一种成本效益高、无毒、环保且简单的方法,利用芦荟叶提取物通过溶胶-凝胶自燃烧法绿色合成CoFe2O4纳米颗粒。为了弄清楚它们的结构、形态和磁性,使用X射线衍射(XRD)、傅立叶变换红外(FTIR)、扫描电子显微镜(SEM)、能量色散X射线光谱(EDX)、透射电子显微镜(TEM)、动态光散射(DLS)、ζ电位和振动样品磁强计(VSM)对合成的纳米颗粒进行了表征。XRD分析表明,颗粒具有单相尖晶石晶体结构,平均晶粒尺寸为33.5 nm。在VSM研究中,所产生的NP表现出软铁磁性。SEM显示,所制备的纳米颗粒由于其磁性行为而聚集。据我们所知,首次全面研究了CoFe2O4纳米粒子对耐药革兰氏阳性菌和革兰氏阴性菌以及真菌菌株的抗癌、抗菌膜、抗菌和抗癌活性。合成的NP具有0.25–0.75的最小抑制浓度 mg/ml对抗测试的病原体。在浓度为0.125–0.5的选定菌株中,CoFe2O4 NPs可抑制37.3–61.8%的生物膜形成 mg/ml。观察到,纳米颗粒不仅抑制了生物膜的形成,而且根除了50.9-64.49%的成熟生物膜,SEM进一步支持了这一点。SEM分析表明,纳米颗粒显著抑制了测试生物膜菌株的定植和聚集。光镜分析显示,NPs完全抑制白色念珠菌菌丝和丝的发育,从而显著减弱其致病性。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)法和4′,6-二脒基-2-苯基吲哚(DAPI)染色表明,NP显著抑制HCT-116和HeLa细胞的增殖。此外,经处理的细胞的SEM图像显示细胞壁褶皱和受损,表明膜破裂和无序。本研究表明,合成的纳米颗粒对耐药细菌念珠菌及其预制生物膜的生长具有有效的抑制作用。因此,这些具有广谱应用的NP可以在医疗环境中被利用,以减少由这些致病菌株引起的基于生物膜的感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioinspired ferromagnetic CoFe2O4 nanoparticles: Potential pharmaceutical and medical applications
Abstract The primary goal of this work was to develop a cost-effective, non-toxic, eco-friendly, and simple approach for the green synthesis of CoFe2O4 nanoparticles (NPs) using Aloe vera leaf extract by the sol–gel auto-combustion method. In order to figure out their structural, morphological, and magnetic properties, the synthesized NPs were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscope (TEM), dynamic light scattering (DLS), zeta potential, and vibrating-sample magnetometer (VSM). XRD analysis showed that particles had a single-phase spinel crystalline structure with an average crystalline size of 33.5 nm. Under VSM studies, the produced NPs exhibit a soft ferromagnetic property. SEM revealed that the as-prepared NPs were agglomerated due to their magnetic behavior. To the best of our knowledge, the anticandidal, antibiofilm, antibacterial, and anticancer activities of CoFe2O4 NPs toward drug-resistant gram-positive and gram-negative bacteria, as well as fungal strains, have been comprehensively investigated for the first time. The synthesized NPs had a minimal inhibitory concentration of 0.25–0.75 mg/ml against the tested pathogens. CoFe2O4 NPs inhibited the biofilm formation by 37.3–61.8% in selected strains at concentrations of 0.125–0.5 mg/ml. It was observed that the NPs not only suppress biofilm formation but also eradicate established mature biofilms by 50.9–64.49% that was further supported by SEM. SEM analysis shows that NPs significantly inhibit the colonization and aggregation of tested biofilm strains. Light microscopic analysis revealed that NPs completely inhibit the development of hyphae and filaments in Candida albicans, which significantly attenuates their pathogenicity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and 4′,6-diamidino-2-phenylindole (DAPI) staining demonstrate that NPs significantly inhibit the proliferation of HCT-116 and HeLa cells. Furthermore, the SEM images of treated cells showed wrinkled and damaged cell walls, indicating the disruption and disorganization of the membrane. This study showed that the synthesized NPs were effective in inhibiting the growth of drug-resistant bacteria, candida, and their preformed biofilms as well. Thus, these NPs with broad-spectrum applications could be exploited in medical settings to diminish biofilm-based infections caused by these pathogenic strains.
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来源期刊
Nanotechnology Reviews
Nanotechnology Reviews CHEMISTRY, MULTIDISCIPLINARY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
11.40
自引率
13.50%
发文量
137
审稿时长
7 weeks
期刊介绍: The bimonthly journal Nanotechnology Reviews provides a platform for scientists and engineers of all involved disciplines to exchange important recent research on fundamental as well as applied aspects. While expert reviews provide a state of the art assessment on a specific topic, research highlight contributions present most recent and novel findings. In addition to technical contributions, Nanotechnology Reviews publishes articles on implications of nanotechnology for society, environment, education, intellectual property, industry, and politics.
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