{"title":"维生素D受体基因多态性(rs10735810)与慢性牙周炎的关系","authors":"Karthikeyan Murthykumar, Radhika Arjunkumar, Vijayashree Priyadharsini Jayaseelan","doi":"10.1111/jicd.12440","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>The aim of the present study was to analyze the association between vitamin D receptor (<i>VDR</i>) (<i>rs10735810</i>) gene polymorphism and chronic periodontitis (CP).</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the <i>FokI</i> region of the <i>VDR</i> gene (<i>rs10735810</i>). The amplicon was further subjected to genotyping using restriction fragment length polymorphism (RFLP) using the <i>FokI</i> enzyme. The genotype obtained based on RFLP pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the χ<sup>2</sup>-test.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The CP group displayed the highest frequency of CT (20%) and TT (6%) genotypes when compared with the control subjects. Allele frequency was found to be similar in both groups. The C allele was found to be predominant in the study population compared with the T allele.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The present study denotes that the <i>VDR</i> polymorphism (<i>rs10735810</i>) is not associated with CP in the study group analyzed.</p>\n </section>\n </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12440","citationCount":"31","resultStr":"{\"title\":\"Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis\",\"authors\":\"Karthikeyan Murthykumar, Radhika Arjunkumar, Vijayashree Priyadharsini Jayaseelan\",\"doi\":\"10.1111/jicd.12440\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>The aim of the present study was to analyze the association between vitamin D receptor (<i>VDR</i>) (<i>rs10735810</i>) gene polymorphism and chronic periodontitis (CP).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the <i>FokI</i> region of the <i>VDR</i> gene (<i>rs10735810</i>). The amplicon was further subjected to genotyping using restriction fragment length polymorphism (RFLP) using the <i>FokI</i> enzyme. The genotype obtained based on RFLP pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the χ<sup>2</sup>-test.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The CP group displayed the highest frequency of CT (20%) and TT (6%) genotypes when compared with the control subjects. Allele frequency was found to be similar in both groups. The C allele was found to be predominant in the study population compared with the T allele.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>The present study denotes that the <i>VDR</i> polymorphism (<i>rs10735810</i>) is not associated with CP in the study group analyzed.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16204,\"journal\":{\"name\":\"Journal of Investigative and Clinical Dentistry\",\"volume\":\"10 4\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-07-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/jicd.12440\",\"citationCount\":\"31\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Investigative and Clinical Dentistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jicd.12440\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Investigative and Clinical Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jicd.12440","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis
Aim
The aim of the present study was to analyze the association between vitamin D receptor (VDR) (rs10735810) gene polymorphism and chronic periodontitis (CP).
Methods
A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the FokI region of the VDR gene (rs10735810). The amplicon was further subjected to genotyping using restriction fragment length polymorphism (RFLP) using the FokI enzyme. The genotype obtained based on RFLP pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the χ2-test.
Results
The CP group displayed the highest frequency of CT (20%) and TT (6%) genotypes when compared with the control subjects. Allele frequency was found to be similar in both groups. The C allele was found to be predominant in the study population compared with the T allele.
Conclusion
The present study denotes that the VDR polymorphism (rs10735810) is not associated with CP in the study group analyzed.
期刊介绍：
Journal of Investigative and Clinical Dentistry (JICD) aims to publish quality, peer reviewed original research and topical reviews on all aspects of investigative and clinical dentistry and craniofacial research, including molecular studies related to oral health and disease. Although international in outlook, the Editor especially encourages papers from the Asia Pacific. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports through an international journal, which will be available free online until 2012. Its scope, therefore, is broad, inclusive and international, but with a particular focus on Asia Pacific. The Editor welcomes manuscripts in the following key thematic areas in oral and maxillofacial sciences: Community Dentistry and Oral Epidemiology, Conservative Dentistry, Dental Biomaterials, Dental Pedagogy, Endodontics and Traumatology, Implant Dentistry, Oral Biosciences, Oral and Maxillofacial Surgery, Oral Medicine, Oral Pathology and Oral Microbiology, Orthodontics, Oral Radiology, Oral Rehabilitation, Paedodontics, Periodontology and Periodontal Medicine.
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