基底外侧杏仁核中IL-10信号对狂饮样乙醇消耗的调节

IF 5.2 3区 医学 Q1 NEUROSCIENCES
Journal of Neuroimmune Pharmacology Pub Date : 2017-06-01 Epub Date: 2016-09-17 DOI:10.1007/s11481-016-9709-2
S Alex Marshall, Kyle H McKnight, Allyson K Blose, Donald T Lysle, Todd E Thiele
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引用次数: 0

摘要

过量摄入乙醇会改变神经免疫系统,尤其会影响中枢神经系统的细胞因子环境。细胞因子失调已被证明是包括酗酒在内的成瘾性行为的基础;然而,许多研究主要侧重于酒精依赖期间的促炎细胞因子谱。目前的研究通过确定过量摄入乙醇对白细胞介素-10(IL-10)和白细胞介素-4(IL-4)活性的影响,在一种被称为 "黑暗中饮酒"(DID)范例的非依赖性暴饮暴食模型中推进了这项研究。此外,还利用定点药理学测试了IL-10调节乙醇消耗的能力。免疫组化分析表明,乙醇使杏仁基底外侧(BLA)的IL-10减少了50%,但对IL-4没有影响。然而,IL-10和IL-4在杏仁核中央(CEA)均未发生变化。酶联免疫吸附试验证实,杏仁核中的IL-10减少了,但血清中的IL-4没有减少,这表明DID范式引起的这种细胞因子的变化仅限于中枢神经系统。最后,向BLA(而非CeA)双侧输注IL-10可减少狂饮和相应的血液乙醇浓度,但不会影响运动活动或焦虑行为相关性。总之,这些数据支持了酗酒会导致特定抗炎细胞因子失调的观点;然而,改善酒精对细胞因子(如 IL-10)的影响可能会被证明是抑制过度饮酒的一种有效疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modulation of Binge-like Ethanol Consumption by IL-10 Signaling in the Basolateral Amygdala.

Excessive ethanol consumption alters the neuroimmune system and particularly impacts the cytokine milieu of the CNS. Cytokine dysregulation has been shown to underlie addictive-like behaviors including alcohol abuse; however, many studies focus primarily on the proinflammatory cytokine profile during alcohol dependence. The current study furthers this research by determining the impact of excessive ethanol consumption on interleukin-10 (IL-10) and interleukin-4 (IL-4) activity in a model of non-dependent binge consumption called the "drinking in the dark" (DID) paradigm. Furthermore, the ability of IL-10 to modulate ethanol consumption was tested using site-directed pharmacology. Immunohistochemistry analyses determined that ethanol decreased IL-10 by 50 % in the basolateral amygdala (BLA) but had no effect on IL-4. Neither IL-10 nor IL-4, however, were altered in the central amygdala (CEA). Enzyme linked immunosorbent assays confirmed that IL-10 was decreased in the amygdala but not in the serum, suggesting changes of this cytokine with the DID paradigm are restricted to the central nervous system. Finally, bilateral infusions of IL-10 into the BLA, but not CeA, reduced binge-like drinking and corresponding blood ethanol concentrations without impacting either locomotor activity or anxiety-like behavioral correlates. Together, these data support the idea that alcohol abuse dysregulates specific anti-inflammatory cytokines; however, ameliorating alcohol-induced effects on cytokines, like IL-10, may prove to be an effective therapy in curbing excessive consumption.

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来源期刊
CiteScore
13.60
自引率
0.00%
发文量
18
审稿时长
6-12 weeks
期刊介绍: The aims of the Journal of Neuroimmune Pharmacology are to promote the dissemination, interest, and exchange of new and important discoveries for the pharmacology and immunology of the nervous system. The aims parallel that of the Society on NeuroImmune Pharmacology by increasing the fundamental understanding of neurologic and neuropsychiatric disorders affected by the immune system or vice versa and towards pharmacologic measures that lead, either to a better understanding of disease mechanisms, or by improving disease outcomes. The scope of JNIP includes all primary works and reviews into the etiology, prevention, and treatment of neuroimmune and nervous system diseases affected by disordered immunity. Original studies serving to define neuroimmune modulation of environmental or endogenous cues such as toxins and drugs of abuse, hormones, and cytokines are welcome. JNIP will serve as a reliable source of interdisciplinary information bridging the fields of pharmacology, immunology, and neuroscience.
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