问题信息

Q1 Health Professions

Current Protocols in Cytometry Pub Date : 2020-12-23 DOI:10.1002/cpcy.61

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引用次数: 0

摘要

封面:在Brzoska等人(http://doi.org/10.1002/cpcy.80)中，图像显示了肺血管通透性的量化。(A)定量荧光活体肺显微镜(qFILM)图像显示，野生型小鼠在激光诱导的组织损伤后，血管内(IV)给药的德克萨斯红葡聚糖(红色)渗漏到空气中。中性粒细胞用绿色表示。箭头表示供血小动脉的血流方向。(B) a中虚线框标记区域的放大视图。三个具有代表性的空气空间(虚线框标记)显示了其中的Texas-red-dextran(红色)的积累，可以通过Texas-red-dextran平均强度随时间的变化来测量(插图)。箭头表示向空气中迁移的中性粒细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Issue Information

Cover: In Brzoska et al. (http://doi.org/10.1002/cpcy.80), the image shows quantification of pulmonary vascular permeability. (A) Quantitative fluorescence intravital lung microscopy (qFILM) image showing vascular leakage of intravascularly (IV) administrated Texas-red-dextran (red) into the air spaces in wild-type mouse following laser-induced tissue damage. Neutrophils shown in green. Arrow shows direction of blood flow in feeding arteriole. (B) Magnified view of region marked with dashed box in A. Three representative air spaces (marked with dashed outline) show accumulation of Texas-red-dextran (red color) inside them, which can be measured as change in mean intensity of Texas-red-dextran over time (inset). Arrows mark neutrophils migrating into the air spaces.

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来源期刊

Current Protocols in Cytometry Health Professions-Medical Laboratory Technology

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期刊介绍： Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.