基于壳聚糖-量子点纳米复合修饰电极的免疫传感器设计，用于临床分析中降钙素原的选择性传感

IF 0.7 Q4 PHARMACOLOGY & PHARMACY

Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI:10.4103/jrptps.JRPTPS_83_20

Sajad Naghizadeh, B. Paknejad, S. Abtahi, E. Hazrati, M. Chamanara

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引用次数: 0

摘要

开发了一种基于壳聚糖（CHI）和量子点（QDs）的简单自由标记电化学免疫传感器，用于检测降钙素原（ProCT）抗原（Ag），该抗原可作为传染病的靶生物标志物。ProCT生物标志物的抗体在CHI QDs平台上成功孵育。电化学阻抗谱和循环伏安法用于评估和表征涂覆在电极上的各种层。在最佳条件下，免疫传感器的电子转移电阻（Rct）随ProCT浓度线性变化。所提出的免疫传感器与0.005至0.35ng/mL的ProCT生物标志物浓度成比例，计算出的检测限为0.015 pg/mL。所提出的生物传感装置还表现出高稳定性和再现性，可用于临床分析中ProCT生物标志物的定量测定。

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Immunosensor design based on Chitosan-QDs, a nanocomposite-modified electrode for selective procalcitonin sensing in clinic analyses

A simple free labeled electrochemical immunosensor based on chitosan (CHI) and quantum dots (QDs) was developed for the determination of procalcitonin (ProCT) antigen (Ag) which can act as a target biomarker of infectious diseases. The antibodies of the ProCT biomarker were successfully incubated on the CHI-QDs platform. Electrochemical impedance spectroscopy and cyclic voltammetry were used for the evaluation and characterization of various layers coated onto the electrode. Under the optimum conditions, the electron transfer resistance (Rct) of the immunosensor was altered linearly by the ProCT concentration. The proposed immunosensor was proportional to the ProCT biomarker concentration from 0.005 to 0.35ng/mL with a calculated detection limit of 0.015 pg/mL. The proposed biosensing device also exhibited high stability and reproducibility, which can be used for the quantitative assay of the ProCT biomarker in clinic analyses.

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来源期刊

Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.