Erina Hilmayanti, N. Nurlelasari, D. G. Katja, A. Hidayat, S. Fajriah, A. Darmawan, U. Supratman, M. N. Azmi, Y. Shiono
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引用次数: 4
摘要
类柠檬酮是三萜化合物的衍生物,由于开环、重排和高度氧化,具有多种结构。类柠檬素是一种具有广泛生物活性的化合物,包括抗癌活性。本研究的目的是测定荔枝果正己烷提取物中类柠檬素的化学结构和细胞毒活性。用甲醇提取香果干粉,再用正己烷、乙酸乙酯和正丁醇进行分馏。从Chisocheton lasiocarpus果实正己烷提取物中分离得到6α-(乙酰氧基)-14β、15β-环氧氮杂二酮(1)、dysobinin(2)、7α-乙酰新三烯酮(3)、6α- o -乙酰-7-去乙酰硝基苯二酚(4)和7α-羟基新三烯酮(5)5种氮杂二酮型柠檬酮。所有化合物的化学结构通过光谱分析,包括1D, 2D-NMR, IR, UV和HR-TOF MS,并与先前报道的光谱数据进行比较。化合物1 ~ 5为首次从该植物中分离得到。结果表明,7α-羟基新三烯酮(5)对MCF-7乳腺癌具有中等的细胞毒活性,IC50值为53 μM
Azadirone-Type Limonoids from the Fruit of Chisocheton lasiocarpus and Their Cytotoxic Activity Against MCF-7 Breast Cancer Lines
Limonoid is derivatives of triterpenoid compound that have a wide variety of structures due to various ring-opening, rearrangements, and high-degree of oxidation. Limonoid is known as compounds that have wide-range of biological activities, including anticancer activity. This research was aimed to determine the chemical structure and cytotoxic activity of limonoid in the n-hexane extract of Chisocheton lasiocarpus fruit. Dried powder of C. lasiocarpus fruit was extracted using methanol followed by fractionated using n-hexane, ethyl acetate, and n-butanol. Five azadirone-type limonoids, 6α-(acetoxy)-14β,15β-epoxyazadirone (1), dysobinin (2), 7α-acetylneotricilenone (3), 6α-O-acetyl-7-deacetylnimocinol (4), and 7α-hyroxyneotricilenone (5), were isolated from the n-hexane extract of Chisocheton lasiocarpus fruit. The chemical structure of all compounds was identified by spectroscopic analysis, including 1D, 2D-NMR, IR, UV and HR-TOF MS as well as by comparison with previous reported spectra data. Compounds 1-5 were investigated from this plant for first time. The cytotoxic activity of the isolated compounds against MCF-7 breast cancer line were examined and the results showed that 7α-hyroxyneotricilenone (5) showed the moderate activity with IC50 values of 53 μM