用随机扩增多态性DNA（RAPD）和单核序列重复序列（ISSR）对李的遗传分析

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2022-04-18 DOI:10.1155/2022/2409324

Jun Li, Guangchun Gao, Bin Li, Bai Li, Qihua Lu

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引用次数: 5

摘要

背景酸梅是一种重要的经济价值果树，主要分布在北半球。方法从中国、日本、美国、新西兰等8个省、自治区、直辖市、直辖市采集李25份。分别用随机扩增多态性DNA（RAPD）和单序列间重复序列（ISSR）技术对这些样品的遗传变异进行了表征，并进行了组合分析。结果共有257个RAPD条带，范围为200～2300 其中81.59%的条带具有多态性。ISSR分析确定了179个波段，范围为300～2500 其中87.74%的条带具有多态性。ISSR结果表明，样品P10（中国安徽麦黄里）与样品P13（中国黑龙江龙源秋里）的相似系数指数最低，而样品P10和样品P15（日本百里）的相似度指数最高。RAPD和ISSR的联合分析表明，样品P4（浙江宁波七皮里）和样品P13（黑龙江龙源秋里）的相似系数指数最低，而样品P19（美国Laroda）和样品P20（美国红心）的相似指数最高。结论RAPD与ISSR分析相结合可用于李属植物的遗传鉴定。

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Genetic Analysis of Prunus salicina L. by Random Amplified Polymorphic DNA (RAPD) and Intersimple Sequence Repeat (ISSR)

Background Prunus salicina L. is an important fruit tree species of great economic value which is mainly distributed in the northern hemisphere. Methods 25 samples of Prunus salicina L. were collected from 8 provinces in China, Japan, USA, and New Zealand. The genetic variations of these samples were characterized by the random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) technique, respectively, and in combination. Results Totally, 257 RAPD bands ranging 200∼2300 bp was found, and 81.59% of these bands were polymorphic. ISSR analysis identified 179 bands ranging 300∼2500 bp, and 87.74% of the bands were polymorphic. ISSR results showed that the similarity coefficient index between samples P10 (Maihuangli in Anhui, Chin) and P13 (Longyuanqiuli in Heilongjiang, China) was lowest, while that between samples P10 (Maihuangli in Anhui, Chin) and P15 (Baili in Japan) was highest. Combined analysis of RAPD and ISSR demonstrated that the similarity coefficient index between samples P4 (Qiepili in Ningbo, Zhejiang, China) and P13 (Longyuanqiuli in Heilongjiang, China) was lowest, while that between samples P19 (Laroda in USA) and P20 (Red heart in USA) was highest. Conclusion RAPD combined with ISSR analysis can be used for genetic characterization of Prunus L. species.

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.