{"title":"mirna-27a以芽生同源物2表达为目标影响退变椎间盘血管的生长","authors":"Ping Wang","doi":"10.31901/24566330.2022/22.03.804","DOIUrl":null,"url":null,"abstract":"This study aimed to evaluate the effects of miRNA-27a-targeting sprouty homolog 2 (SPRY2) on nucleus pulposus cell (NPC)-induced angiogenesis of human microvascular endothelial cells (HMEC-1) in degenerated intervertebral disc. Intervertebral disc tissues were collected from patients with scoliosis (control) and intervertebral disc degeneration (IDD). HMEC-1 cells were divided into control, negative control, sh-miR-27a, miR-27a, SPRY2 and miR-27a + SPRY2 groups. The invasive and angiogenic abilities of HMEC-1 cells were detected through Transwell and tube formation assays. TGF-â1 levels in NPCs and mixed medium were detected by enzyme-linked immunosorbent assay. MiR-27a expression in the intervertebral disc tissue of IDD group significantly exceeded that of the control group. In the SPRY2 group, the number of invading HMEC-1 cells decreased (P<0.05). Compared with the miR-27a group, the miR-27a + SPRY2 group had weakened invasive and angiogenic abilities, and decreased TGF-â1 expression (P<0.05). MiR-27a promotes NPC-induced angiogenesis of HMEC-1 cells through targeted inhibition of SPRY2 expression.","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"mirna-27a Targets Sprouty Homolog 2 Expression to Affect Growth of Blood Vessels into Degenerated Intervertebral Disc\",\"authors\":\"Ping Wang\",\"doi\":\"10.31901/24566330.2022/22.03.804\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aimed to evaluate the effects of miRNA-27a-targeting sprouty homolog 2 (SPRY2) on nucleus pulposus cell (NPC)-induced angiogenesis of human microvascular endothelial cells (HMEC-1) in degenerated intervertebral disc. Intervertebral disc tissues were collected from patients with scoliosis (control) and intervertebral disc degeneration (IDD). HMEC-1 cells were divided into control, negative control, sh-miR-27a, miR-27a, SPRY2 and miR-27a + SPRY2 groups. The invasive and angiogenic abilities of HMEC-1 cells were detected through Transwell and tube formation assays. TGF-â1 levels in NPCs and mixed medium were detected by enzyme-linked immunosorbent assay. MiR-27a expression in the intervertebral disc tissue of IDD group significantly exceeded that of the control group. In the SPRY2 group, the number of invading HMEC-1 cells decreased (P<0.05). Compared with the miR-27a group, the miR-27a + SPRY2 group had weakened invasive and angiogenic abilities, and decreased TGF-â1 expression (P<0.05). MiR-27a promotes NPC-induced angiogenesis of HMEC-1 cells through targeted inhibition of SPRY2 expression.\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.31901/24566330.2022/22.03.804\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31901/24566330.2022/22.03.804","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
mirna-27a Targets Sprouty Homolog 2 Expression to Affect Growth of Blood Vessels into Degenerated Intervertebral Disc
This study aimed to evaluate the effects of miRNA-27a-targeting sprouty homolog 2 (SPRY2) on nucleus pulposus cell (NPC)-induced angiogenesis of human microvascular endothelial cells (HMEC-1) in degenerated intervertebral disc. Intervertebral disc tissues were collected from patients with scoliosis (control) and intervertebral disc degeneration (IDD). HMEC-1 cells were divided into control, negative control, sh-miR-27a, miR-27a, SPRY2 and miR-27a + SPRY2 groups. The invasive and angiogenic abilities of HMEC-1 cells were detected through Transwell and tube formation assays. TGF-â1 levels in NPCs and mixed medium were detected by enzyme-linked immunosorbent assay. MiR-27a expression in the intervertebral disc tissue of IDD group significantly exceeded that of the control group. In the SPRY2 group, the number of invading HMEC-1 cells decreased (P<0.05). Compared with the miR-27a group, the miR-27a + SPRY2 group had weakened invasive and angiogenic abilities, and decreased TGF-â1 expression (P<0.05). MiR-27a promotes NPC-induced angiogenesis of HMEC-1 cells through targeted inhibition of SPRY2 expression.