Tiphane Andrade Figueira, A. Silva, A. Enrich-Prast, Y. Yoneshigue-Valentin, V. Oliveira
{"title":"从栽培和采集的羽扇Ulva(叶绿素a)中提取的Ulvan多糖的结构特征","authors":"Tiphane Andrade Figueira, A. Silva, A. Enrich-Prast, Y. Yoneshigue-Valentin, V. Oliveira","doi":"10.4236/abb.2020.115016","DOIUrl":null,"url":null,"abstract":"Ulvan is a sulfated heteropolysaccharide present in \nthe cell wall of Ulva species with \nunique structural properties and technological potential. Here we characterized \nby FTIR and NMR analysis the structure of ulvan from Ulva fasciata collected in natural environment (SEA) and after in vitro biomass cultivation in \nnutrient enriched water (CULT). FTIR spectrum of CULT ulvan presented stronger \nsignals of sulfate groups than SEA. 1H and 13C NMR showed \nthat both ulvan are composed mainly of ulvanobiuronic acid 3-sulfate type A and \ntype B. SEA ulvan presented signals characteristics of xylose, suggesting the \npresence of ulvanobiose in its structure, while CULT presented most signals of \ntype A disaccharide. The cultivation of Ulva could be an alternative to suffice the emerging demand for ulvan meeting requirements \nof quality and quantity.","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"21","resultStr":"{\"title\":\"Structural Characterization of Ulvan Polysaccharide from Cultivated and Collected Ulva fasciata (Chlorophyta)\",\"authors\":\"Tiphane Andrade Figueira, A. Silva, A. Enrich-Prast, Y. Yoneshigue-Valentin, V. Oliveira\",\"doi\":\"10.4236/abb.2020.115016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Ulvan is a sulfated heteropolysaccharide present in \\nthe cell wall of Ulva species with \\nunique structural properties and technological potential. Here we characterized \\nby FTIR and NMR analysis the structure of ulvan from Ulva fasciata collected in natural environment (SEA) and after in vitro biomass cultivation in \\nnutrient enriched water (CULT). FTIR spectrum of CULT ulvan presented stronger \\nsignals of sulfate groups than SEA. 1H and 13C NMR showed \\nthat both ulvan are composed mainly of ulvanobiuronic acid 3-sulfate type A and \\ntype B. SEA ulvan presented signals characteristics of xylose, suggesting the \\npresence of ulvanobiose in its structure, while CULT presented most signals of \\ntype A disaccharide. The cultivation of Ulva could be an alternative to suffice the emerging demand for ulvan meeting requirements \\nof quality and quantity.\",\"PeriodicalId\":65405,\"journal\":{\"name\":\"生命科学与技术进展(英文)\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-05-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"生命科学与技术进展(英文)\",\"FirstCategoryId\":\"1089\",\"ListUrlMain\":\"https://doi.org/10.4236/abb.2020.115016\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"生命科学与技术进展(英文)","FirstCategoryId":"1089","ListUrlMain":"https://doi.org/10.4236/abb.2020.115016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Structural Characterization of Ulvan Polysaccharide from Cultivated and Collected Ulva fasciata (Chlorophyta)
Ulvan is a sulfated heteropolysaccharide present in
the cell wall of Ulva species with
unique structural properties and technological potential. Here we characterized
by FTIR and NMR analysis the structure of ulvan from Ulva fasciata collected in natural environment (SEA) and after in vitro biomass cultivation in
nutrient enriched water (CULT). FTIR spectrum of CULT ulvan presented stronger
signals of sulfate groups than SEA. 1H and 13C NMR showed
that both ulvan are composed mainly of ulvanobiuronic acid 3-sulfate type A and
type B. SEA ulvan presented signals characteristics of xylose, suggesting the
presence of ulvanobiose in its structure, while CULT presented most signals of
type A disaccharide. The cultivation of Ulva could be an alternative to suffice the emerging demand for ulvan meeting requirements
of quality and quantity.