纳米羟基磷灰石/壳聚糖复合支架培养成骨样细胞治疗骨缺损及活动受限的研究

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
A. Emamgholi, M. Rahimi, S. Tabaei, Teymoor Ghorbani, S. Ziai, P. B. Milan, Mahdi Khodadoust, Nafiseh Keshavarzian
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引用次数: 3

摘要

目的:设计和构建生物相容性和可生物降解的支架是组织工程的主要目标之一。近年来,纳米羟基磷灰石作为一种与人体骨组织矿物相高度相似的生物活性生物陶瓷剂,与生物可降解聚合物和种植体涂层结合使用,引起了生物材料科学领域研究人员的关注。本研究旨在研究壳聚糖/聚氧聚乙烯/纳米羟基磷灰石支架对成年大鼠骨髓基质细胞(BMSCs)向成骨样细胞分化的影响。材料与方法:制备壳聚糖和PEO溶液,质量比分别为80:20和70:30,加入2%重量的纳米羟基磷灰石。采用静电纺丝法制备了纳米纤维,并用扫描电镜对其形貌进行了研究。将成熟大鼠骨髓间充质干细胞在纳米纤维上培养,加入分化培养基进行分化。在第1、2、3周末用吖啶橙染色评价分化细胞的存活情况,用扫描电镜观察纳米纤维对分化细胞的影响。结果:纳米纤维的平均直径为150±17纳米,比例为80:20。茜素红染色证实骨髓间充质干细胞在纳米纤维上向成骨细胞样细胞分化。结果表明,在分化的第三周结束时,与对照组相比,纳米纤维组的分化细胞存活率显著降低。结论:在含有纳米羟基磷灰石的壳聚糖/PEO纳米纤维的作用下,骨髓间充质干细胞可向成骨细胞样细胞分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of osteoblast-like cells cultured on nano-hydroxyapatite/chitosan based composite scaffold in the treatment of bone defects and limited mobility
Objective(s): Design and construction of biocompatible and biodegradable scaffolds are among the main goals of tissue engineering. Recently, use of nano-hydroxyapatite as a bioactive bioceramic agent with high similarity to the mineral phase of the human bone tissue, in combination with biodegradable polymers and implant coatings has attracted the attention of researchers in the field of biomaterial sciences. The present study aimed to assess the differentiation of bone marrow stromal cells (BMSCs) in osteoblast-like cells on the chitosan/polyethylene oxide (PEO)/nano-hydroxyapatite scaffold in mature rats.Materials and Methods: Chitosan and PEO solution with the weight ratio of 80:20 and 70:30 were prepared, and 2% weight of nano-hydroxyapatite was added. Nanofibers were prepared using the electrospinning method, and the morphology was studied using scanning electron microscopy (SEM). Afterwards, the BMSCs of mature rats were cultured on nanofibers and differentiated by adding a differentiation medium. The survival of the differentiated cells was evaluated at the end of the first, second, and third week using acridine orange staining, and the morphology of the differentiated cells exposed to nanofibers was assessed using SEM. Results: The mean diameter of the nanofibers with the ratio of 80:20 was 150±17 nanometers. The differentiation of BMSCs into the osteoblast-like cells on nanofibers was confirmed using Alizarin red staining. The results indicated a significant decrease in the survival of the differentiated cells in the nanofiber groups by the end of the third week of differentiation compared to the control samples.Conclusion: According to the results, BMSCs could be differentiated into osteoblast-like cells in the presence of the chitosan/PEO nanofibers containing nano-hydroxyapatite.
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
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12 weeks
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