气味结合蛋白基因和化学感觉蛋白基因在褐金龟中的表达分析(鞘翅目:金龟科)

IF 0.2 4区 农林科学 Q4 ENTOMOLOGY
Haoliang Chen, M. Qu, F. Ali, Lulu Lin, Ming-hui Xie, Guangling Zhang, W. Su
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引用次数: 3

摘要

摘要:扁蚧是一种在我国广泛分布的害虫,在农业、园艺、林业等领域都会造成巨大的经济损失。它很难控制,因为生命周期的大部分都在地下。诱捕成虫将是一种环境可持续的方式,可以减少这种昆虫造成的损害。气味结合蛋白(OBPs)和化学感受蛋白(CSP)被认为是重要的化学检测蛋白。在这项研究中,我们研究了气味结合蛋白基因和化学感受蛋白基因在成年和其他发育阶段不同组织中的表达。除了OBP7在没有天线的头部中表达最高外,天线对所有组织中的OBPs和CSP的表达水平最高。除天线外,没有天线的头部的所有OBP和CSP基因的表达水平次之。成人中OBP2、OBP3、OBP14和CSP的表达高于其他阶段,而OBP7的表达低于其他阶段。在估计OBPs和CSP的表达之前,测试了7个候选参考基因在成年和其他发育阶段组织中的稳定性。GAPDH是成虫不同组织中最佳的参考基因,RPL13a是不同发育阶段的最佳参考基因。我们的研究结果为进一步研究这种昆虫化学通讯的分子方面提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression Analysis of Odorant-Binding Protein Genes and Chemosensory Protein Genes in Anomala corpulenta Motschulsky (Coleoptera: Scarabaeidae)
ABSTRACT: Anomala corpulenta is an insect pest that is widely distributed in China, and it can cause large economic losses in agriculture, horticulture, and forestry. It is difficult to control because most of the lifecycle is underground. Trapping adults would be an environmentally sustainable way to reduce damage caused by this insect. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are considered to be important chemodetection proteins. In this study, we investigate the expression of odorant-binding protein genes and chemosensory protein genes in different tissues of the adult and other developmental stages. The antenna had the highest expression levels for OBPs and CSPs of all tissues, except for OBP7 which was most highly expressed in the head without antenna. Besides the antenna, the head without antenna had the next highest expression levels for all OBP and CSP genes. The expression of OBP2, OBP3, OBP14, and CSP in the adult was higher than in other stages, while, OBP7 was lower than in other stages. Before estimating the expression of OBPs and CSPs, seven candidate reference genes were tested for stability among the tissues of adult and other development stages. GAPDH was the best reference gene in different tissues of the adult, and RPL13a was the best reference gene in different development stages. Our results provide a foundation for further research on the molecular aspects of chemical communication of this insect.
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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
12
审稿时长
>12 weeks
期刊介绍: The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.
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