紫锥菊精油对黄热病斑马鱼(Danio Rerio)血液学参数和肠道微生物群的影响

Q4 Veterinary
Tina Hasankhani, D. Nikaein, A. Khosravi, H. Rahmati-Holasoo, Mona Hasankhany
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引用次数: 0

摘要

背景:黄曲霉毒素是最重要的真菌毒素之一,具有肝毒性、致畸性和免疫抑制特性。这种霉菌毒素主要由饲料中的曲霉菌属产生。因此,在不影响饲料质量的情况下,使用能够预防黄曲霉毒素并发症的化合物是非常有益的。目的:研究紫锥菊精油对黄曲霉毒素喂养的斑马鱼胃肠道微生物群及部分血液因子的调节作用。方法:将斑马鱼分为4组,每组45尾,分3个重复:对照组(G1);G2,饲料中含有500µg/kg紫荆精油;G3,饲料中添加500µg/kg紫荆精油和3ppm黄曲霉毒素B1(AFB1);G4,饲料中AFB1含量为3ppm。饲喂饲料60天。在这段时间后,对它们实施安乐死,从尾静脉采血,并准备血液涂片。用自动分析仪测定鱼肝胰脏中丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶的含量。同时,培养肠道内容物以评估微生物种群。结果:结果显示,黄曲霉毒素组肝酶升高(P<0.05),与AFB1组相比,黄曲霉精油与AFB1同时使用降低了肝酶。此外,AFB1可以将微生物群体转化为病原体。G2组和G3组的差异血球计数显示中性粒细胞和血小板百分比增加。结论:紫荆挥发油可减轻斑马鱼慢性AFB1污染的不良反应。尽管如此,紫脓杆菌在斑马鱼体内的免疫功能及其对AFB1的作用机制还有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Echinacea Purpurea L. (Eastern Purple Coneflower) Essential Oil on Hematological Parameters and Gut Microbial Population of Zebrafish (Danio Rerio) With Aflatoxicosis
Background: Aflatoxin is one of the most important fungal toxins with documented hepatotoxic, teratogenic, and immunosuppressive properties. This mycotoxin is mainly produced by species of the genus Aspergillus in feed. Therefore, the application of compounds, which prevent complications of aflatoxins without losing feed quality, is highly beneficial. Objectives: This study aimed to determine the effect of the Echinacea purpurea L. essential oils on the regulation of the microbial population of the gastrointestinal tract and some blood factors of aflatoxin-fed zebrafish. Methods: Zebrafish were divided into four groups of 45 fish in three replicates: control (G1); G2, fish fed with feed containing 500 µg/kg E. purpurea L. essential oils; G3, fish fed with feed containing 500 µg/kg E. purpurea L. essential oil and 3 ppm aflatoxin B1(AFB1); and G4, fish fed with feed containing 3 ppm AFB1. The fish were fed with diets for 60 days. After this period, they were euthanized, blood was collected from the tail vein, and blood smears were prepared. Fish hepatopancreas were used to measure alanine aminotransferase, aspartate transaminase, and alkaline phosphatase enzymes with an auto-analyzer. Also, intestinal contents were cultured to evaluate microbial population. Results: Results showed that liver enzymes increased in the aflatoxin group (P<0.05), and concurrent use of the essential oil along with AFB1 reduced the liver enzymes compared with the AFB1-treated group. Moreover, AFB1 could convert the microbial population to pathogens. Differential blood counts in the G2 and G3 groups showed an increase in the percentages of neutrophils and thrombocytes. Conclusion: According to the results of this study, E. purpurea L. essential oils could reduce the adverse effects of chronic contamination with AFB1 in zebrafish. Nevertheless, more studies are needed to better understand the immunological function of E. purpurea L. in zebrafish and its mechanism of action against AFB1.
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来源期刊
Iranian Journal of Veterinary Medicine
Iranian Journal of Veterinary Medicine Veterinary-General Veterinary
CiteScore
0.90
自引率
0.00%
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审稿时长
6 weeks
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