基于元素探针的CRISPR/Cas12a生物传感技术对妥布霉素的敏感性检测

IF 3.4 2区化学 Q1 SPECTROSCOPY

Atomic Spectroscopy Pub Date : 2022-01-25 DOI:10.46770/as.2021.1013

R. Liu

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引用次数: 2

摘要

：CRISPR/Cas（聚集的规则间隔短回文重复序列相关蛋白）的精确靶向性和优异的特异性切割能力引起了人们的广泛研究兴趣。除了核酸检测，研究人员还在探索该系统检测各种其他目标的潜力。在此，我们提出了一种新的基于CRISPR/Cas生物传感的妥布霉素（TOB）检测方法。结合适体识别和元件标记策略，实现了TOB的高灵敏度和特异性检测。由于电感耦合等离子体质谱法（ICP-MS）强大的元素分析能力和CRISPR/Cas优异的信号放大效果，该方法中TOB的线性浓度范围为2.8-100nM，而检测限为0.83nM。该方法已成功应用于实际水样的分析。

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Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection

: The precise targeting and excellent specific cleave capability of CRISPR/Cas (clustered regularly inters-paced short palindromic repeats-associated proteins) have gained much research interest. In addition to nucleic acid detection, researchers exploring the potential of this system for detecting various other targets. Herein, we proposed a novel CRISPR/Cas biosensing based Tobramycin (TOB) detection method. Combining with the aptamer recognition and element labeling strategy, highly sensitive and specific detection of TOB is realized. Due to the powerful elemental analysis capabilities of inductively coupled plasma mass spectrometry (ICP-MS) and the excellent signal amplification effect of CRISPR/Cas, the linear concentration range for TOB was 2.8-100 nM in this method, whereas the detection limit was 0.83 nM. The proposed method has been successfully applied in the analysis of actual water samples.

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来源期刊

Atomic Spectroscopy 物理-光谱学

CiteScore

5.30

自引率

14.70%

发文量

审稿时长

4.5 months

期刊介绍： The ATOMIC SPECTROSCOPY is a peer-reviewed international journal started in 1962 by Dr. Walter Slavin and now is published by Atomic Spectroscopy Press Limited (ASPL). It is intended for the rapid publication of both original articles and review articles in the fields of AAS, AFS, ICP-OES, ICP-MS, GD-MS, TIMS, SIMS, AMS, LIBS, XRF and related techniques. Manuscripts dealing with (i) instrumentation & fundamentals, (ii) methodology development & applications, and (iii) standard reference materials (SRMs) development can be submitted for publication.