Effects of combined Garcinia kola and Kigelia africana on Insulin and Paraoxonase 1 (PON1) levels in type 2 diabetic rats.

BACKGROUND Several reports of individual extracts of Garcina kola and Kigelia africana beneficial effects against several factors related with development of diabetes mellitus abound. However, there is still lack of information about the combined effects of these extracts on Insulin and Paraoxonase 1 (PON-1) in Streptozotocin-Nicotinamide induced type-2 diabetic Wistar rats. METHODS Forty-two young male rats (180-200g) were randomly divided into six groups (n=7/group). Diabetes was intraperitoneally induced with 110 mg/kg of nicotinamide constituted in distilled water andfifteen minutes after with 65mg/kg of streptozocin freshly prepared in 0.1M citrate buffer (pH of 4.5) and treated for six weeks as follows: the control rats received either 0.9% normal saline (NS) or 250mg/kg extract by gavage. The remaining animals were diabetic induced and subsequently treated with either NS, graded doses of the extract (250mg/kg and 500mg/kg) or 5mg/kg Glibenclamide + 100mg/kg Metformin. Gas chromatography mass spectrometry (GCMS) of the combined extracts was also analyzed to identify the bioactive compounds present. Insulin, PON-1 levels, lipid profiles and atherogenic index were assessed. RESULTS Our findings show that Insulin and PON-1 levels in the plasma of diabetic rats treated with the combined extracts were significantly increased when compared to the control rats. Moreover, the GCMS of the extract show the presence of both monosaturated (oleic acid) and polyunsaturated (linoleic acid) fatty acids. CONCLUSION The current findings suggest that the extract may help improve glucose homeostasis, prevent atherosclerosis through established mechanism of the identified bioactive compounds.