参与免疫系统稳态和造血器官发育的新分子标记物在卵母细胞体外成熟过程中受到不同的调节

Q4 Biochemistry, Genetics and Molecular Biology
Lisa Moncrieff, I. Kocherova, A. Bryja, W. Kranc, Joanna Perek, P. Celichowski, M. Kulus, B. Kempisty, P. Mozdziak, M. Ješeta
{"title":"参与免疫系统稳态和造血器官发育的新分子标记物在卵母细胞体外成熟过程中受到不同的调节","authors":"Lisa Moncrieff, I. Kocherova, A. Bryja, W. Kranc, Joanna Perek, P. Celichowski, M. Kulus, B. Kempisty, P. Mozdziak, M. Ješeta","doi":"10.2478/acb-2020-0004","DOIUrl":null,"url":null,"abstract":"Abstract The growth and maturation of the oocyte is a dynamic process which requires a variable supply of hormones, growth factors and energy. These needs are met partially by the surrounding somatic cells and the cumulus-oocyte complex, which communicate bi-directionally via gap junctions. Identifying and analyzing protein expression in the oocyte can provide insight in its development and growth. Further, like bone marrow stem cells, if relevant marker genes are found in oocytes, there is a potential for the oocyte to be manipulated into becoming hemopoietic stem cells. In this study, porcine oocytes were isolated and subjected to microarray analysis to compare the oocyte gene expression in vivo and in vitro maturation (IVM). Genes identified belonged to both ‘hemopoietic or lymphoid organ development’(GO:0048534) and ‘immune system development’ (GO:0002520), and the markers can be used to identify several activities such as cell migration, neurogenesis and proliferation. The following are the identified genes and all were downregulated after IVM to varying degrees: ID2, VEGFA, TGFBR3, INHBA, CDK6, BCL11A, MYO1E, ITGB1, EGR1, NOTCH2, SPTA1, KIT and TPD52. Our results should provide new markers to further investigate oocyte development and growth regulation. Running title: Markers of hemopoietic organ development","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"8 1","pages":"35 - 43"},"PeriodicalIF":0.0000,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"New molecular markers involved in immune system homeostasis and hemopoietic organ development are differentially regulated during oocytes in vitro maturation\",\"authors\":\"Lisa Moncrieff, I. Kocherova, A. Bryja, W. Kranc, Joanna Perek, P. Celichowski, M. Kulus, B. Kempisty, P. Mozdziak, M. Ješeta\",\"doi\":\"10.2478/acb-2020-0004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract The growth and maturation of the oocyte is a dynamic process which requires a variable supply of hormones, growth factors and energy. These needs are met partially by the surrounding somatic cells and the cumulus-oocyte complex, which communicate bi-directionally via gap junctions. Identifying and analyzing protein expression in the oocyte can provide insight in its development and growth. Further, like bone marrow stem cells, if relevant marker genes are found in oocytes, there is a potential for the oocyte to be manipulated into becoming hemopoietic stem cells. In this study, porcine oocytes were isolated and subjected to microarray analysis to compare the oocyte gene expression in vivo and in vitro maturation (IVM). Genes identified belonged to both ‘hemopoietic or lymphoid organ development’(GO:0048534) and ‘immune system development’ (GO:0002520), and the markers can be used to identify several activities such as cell migration, neurogenesis and proliferation. The following are the identified genes and all were downregulated after IVM to varying degrees: ID2, VEGFA, TGFBR3, INHBA, CDK6, BCL11A, MYO1E, ITGB1, EGR1, NOTCH2, SPTA1, KIT and TPD52. Our results should provide new markers to further investigate oocyte development and growth regulation. Running title: Markers of hemopoietic organ development\",\"PeriodicalId\":18329,\"journal\":{\"name\":\"Medical Journal of Cell Biology\",\"volume\":\"8 1\",\"pages\":\"35 - 43\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medical Journal of Cell Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/acb-2020-0004\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical Journal of Cell Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/acb-2020-0004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0

摘要

摘要卵母细胞的生长和成熟是一个动态过程,需要不同的激素、生长因子和能量供应。这些需求部分由周围的体细胞和卵丘-卵母细胞复合体满足,它们通过间隙连接进行双向交流。识别和分析卵母细胞中的蛋白质表达可以深入了解其发育和生长。此外,与骨髓干细胞一样,如果在卵母细胞中发现相关标记基因,则卵母细胞有可能被操纵成为造血干细胞。在本研究中,分离猪卵母细胞并进行微阵列分析,以比较卵母细胞在体内和体外成熟(IVM)中的基因表达。已鉴定的基因属于“造血或淋巴器官发育”(GO:00048534)和“免疫系统发育”(GO:0002520),这些标记物可用于鉴定细胞迁移、神经发生和增殖等多种活动。以下是已鉴定的基因,它们在IVM后均不同程度下调:ID2、VEGFA、TGFBR3、INHBA、CDK6、BCL11A、MYO1E、ITGB1、EGR1、NOTCH2、SPTA1、KIT和TPD52。我们的研究结果应该为进一步研究卵母细胞的发育和生长调控提供新的标志物。运行标题:造血器官发育标志物
本文章由计算机程序翻译,如有差异,请以英文原文为准。
New molecular markers involved in immune system homeostasis and hemopoietic organ development are differentially regulated during oocytes in vitro maturation
Abstract The growth and maturation of the oocyte is a dynamic process which requires a variable supply of hormones, growth factors and energy. These needs are met partially by the surrounding somatic cells and the cumulus-oocyte complex, which communicate bi-directionally via gap junctions. Identifying and analyzing protein expression in the oocyte can provide insight in its development and growth. Further, like bone marrow stem cells, if relevant marker genes are found in oocytes, there is a potential for the oocyte to be manipulated into becoming hemopoietic stem cells. In this study, porcine oocytes were isolated and subjected to microarray analysis to compare the oocyte gene expression in vivo and in vitro maturation (IVM). Genes identified belonged to both ‘hemopoietic or lymphoid organ development’(GO:0048534) and ‘immune system development’ (GO:0002520), and the markers can be used to identify several activities such as cell migration, neurogenesis and proliferation. The following are the identified genes and all were downregulated after IVM to varying degrees: ID2, VEGFA, TGFBR3, INHBA, CDK6, BCL11A, MYO1E, ITGB1, EGR1, NOTCH2, SPTA1, KIT and TPD52. Our results should provide new markers to further investigate oocyte development and growth regulation. Running title: Markers of hemopoietic organ development
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Medical Journal of Cell Biology
Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
自引率
0.00%
发文量
15
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信