纳米金(AuNP)基青霉素G比色传感器的研制

IF 0.9 Q3 ENGINEERING, MULTIDISCIPLINARY
Darmawati Darmawati, A. Z. Mustopa, B. Budiarto, S. Rahmawati, F. Izzati, R. Harmoko, E. Saepudin, A. Mahsunah
{"title":"纳米金(AuNP)基青霉素G比色传感器的研制","authors":"Darmawati Darmawati, A. Z. Mustopa, B. Budiarto, S. Rahmawati, F. Izzati, R. Harmoko, E. Saepudin, A. Mahsunah","doi":"10.5614/j.eng.technol.sci.2022.54.4.13","DOIUrl":null,"url":null,"abstract":"Antibiotics are chemical or biological substances that have the ability to kill pathogens selectively. Currently, high-performance liquid chromatography (HPLC) is used routinely in the detection of antibiotics. However, the cost of analysis and running time are bottlenecks for HPLC to be used for routine tests to detect antibiotics. Alternative methods need to be developed to overcome this issue. In this study, the development of a penicillin G specific biosensor by using a DNA aptamer and gold nanoparticles (AuNPs) was done. Optimal aptasensor conditions were achieved with the concentrations of NaCl and aptamer at 0.25 M and 2 μM, respectively. An aptasensor of this type showed LOD for penicillin G at 3 mg/L and was able to detect penicillin G in the range of 3 to 27 mg/L. The established aptasensor showed specific sensitivity toward penicillin G after testing with several antibiotics, i.e., ampicillin, kanamycin, chloramphenicol, and erythromycin. The aptasensor could detect the presence of penicillin G from culture medium of wild-type, ultraviolet irradiation mutant, gamma irradiation mutant, and ultraviolet irradiation and gamma irradiation mutant strains of P. chrysogenum, at detection concentrations of 9.75 ± 0.004; 25.25 ± 0.005; 37.5 ± 0.005; and 45 ± 0.004 mg/L, respectively.","PeriodicalId":15689,"journal":{"name":"Journal of Engineering and Technological Sciences","volume":" ","pages":""},"PeriodicalIF":0.9000,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of Gold Nanoparticle (AuNP)-based Colorimetric Aptasensor for Penicillin G Detection\",\"authors\":\"Darmawati Darmawati, A. Z. Mustopa, B. Budiarto, S. Rahmawati, F. Izzati, R. Harmoko, E. Saepudin, A. Mahsunah\",\"doi\":\"10.5614/j.eng.technol.sci.2022.54.4.13\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Antibiotics are chemical or biological substances that have the ability to kill pathogens selectively. Currently, high-performance liquid chromatography (HPLC) is used routinely in the detection of antibiotics. However, the cost of analysis and running time are bottlenecks for HPLC to be used for routine tests to detect antibiotics. Alternative methods need to be developed to overcome this issue. In this study, the development of a penicillin G specific biosensor by using a DNA aptamer and gold nanoparticles (AuNPs) was done. Optimal aptasensor conditions were achieved with the concentrations of NaCl and aptamer at 0.25 M and 2 μM, respectively. An aptasensor of this type showed LOD for penicillin G at 3 mg/L and was able to detect penicillin G in the range of 3 to 27 mg/L. The established aptasensor showed specific sensitivity toward penicillin G after testing with several antibiotics, i.e., ampicillin, kanamycin, chloramphenicol, and erythromycin. The aptasensor could detect the presence of penicillin G from culture medium of wild-type, ultraviolet irradiation mutant, gamma irradiation mutant, and ultraviolet irradiation and gamma irradiation mutant strains of P. chrysogenum, at detection concentrations of 9.75 ± 0.004; 25.25 ± 0.005; 37.5 ± 0.005; and 45 ± 0.004 mg/L, respectively.\",\"PeriodicalId\":15689,\"journal\":{\"name\":\"Journal of Engineering and Technological Sciences\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2022-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Engineering and Technological Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5614/j.eng.technol.sci.2022.54.4.13\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Engineering and Technological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5614/j.eng.technol.sci.2022.54.4.13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

抗生素是具有选择性杀死病原体能力的化学或生物物质。目前,高效液相色谱法是抗生素检测的常用方法。然而,分析成本和运行时间是制约高效液相色谱用于常规检测抗生素的瓶颈。需要开发替代方法来克服这个问题。本研究利用DNA适体和金纳米粒子(AuNPs)制备青霉素G特异性生物传感器。适体浓度为0.25 M和2 μM时,适体传感器的最佳条件为NaCl和适体。该型配体传感器对青霉素G的检出限为3 mg/L,对青霉素G的检出范围为3 ~ 27 mg/L。经氨苄西林、卡那霉素、氯霉素、红霉素等多种抗生素检测,所建立的适体传感器对青霉素G具有特异性敏感性。该传感器能检测出野生型、紫外辐照突变株、γ辐照突变株、紫外辐照和γ辐照突变株培养基中青霉素G的存在,检测浓度为9.75±0.004;25.25±0.005;37.5±0.005;分别为45±0.004 mg/L。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of Gold Nanoparticle (AuNP)-based Colorimetric Aptasensor for Penicillin G Detection
Antibiotics are chemical or biological substances that have the ability to kill pathogens selectively. Currently, high-performance liquid chromatography (HPLC) is used routinely in the detection of antibiotics. However, the cost of analysis and running time are bottlenecks for HPLC to be used for routine tests to detect antibiotics. Alternative methods need to be developed to overcome this issue. In this study, the development of a penicillin G specific biosensor by using a DNA aptamer and gold nanoparticles (AuNPs) was done. Optimal aptasensor conditions were achieved with the concentrations of NaCl and aptamer at 0.25 M and 2 μM, respectively. An aptasensor of this type showed LOD for penicillin G at 3 mg/L and was able to detect penicillin G in the range of 3 to 27 mg/L. The established aptasensor showed specific sensitivity toward penicillin G after testing with several antibiotics, i.e., ampicillin, kanamycin, chloramphenicol, and erythromycin. The aptasensor could detect the presence of penicillin G from culture medium of wild-type, ultraviolet irradiation mutant, gamma irradiation mutant, and ultraviolet irradiation and gamma irradiation mutant strains of P. chrysogenum, at detection concentrations of 9.75 ± 0.004; 25.25 ± 0.005; 37.5 ± 0.005; and 45 ± 0.004 mg/L, respectively.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Engineering and Technological Sciences
Journal of Engineering and Technological Sciences ENGINEERING, MULTIDISCIPLINARY-
CiteScore
2.30
自引率
11.10%
发文量
77
审稿时长
24 weeks
期刊介绍: Journal of Engineering and Technological Sciences welcomes full research articles in the area of Engineering Sciences from the following subject areas: Aerospace Engineering, Biotechnology, Chemical Engineering, Civil Engineering, Electrical Engineering, Engineering Physics, Environmental Engineering, Industrial Engineering, Information Engineering, Mechanical Engineering, Material Science and Engineering, Manufacturing Processes, Microelectronics, Mining Engineering, Petroleum Engineering, and other application of physical, biological, chemical and mathematical sciences in engineering. Authors are invited to submit articles that have not been published previously and are not under consideration elsewhere.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信