常绿无性系矮灌木报春科紫苏微卫星标记的开发与鉴定

IF 1.3 4区农林科学 Q3 FORESTRY

Journal of Forest Research Pub Date : 2022-11-27 DOI:10.1080/13416979.2022.2149058

Atsushi Minami, H. Sugawara, T. Nishimura

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引用次数: 0

摘要

摘要：首次为报春科（Ardisia japonica）开发了微卫星标记，报春科是一种生长在东亚各种温带森林下层的常绿克隆灌木。在基于基因组序列数据设计的69对引物中，有13对在来自两个群体的分株中显示出明显的微卫星峰和具有四倍体的等位基因多态性。使用微卫星标记成功地将Ramets分配到多焦点谱系（基因）。每个基因座的等位基因数在3到8之间，观察到的杂合度在0.468到0.881之间。这将有助于林下无性系灌木的基因分配和基因水平的群体遗传学和生态学研究。

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Development and characterization of microsatellite markers for Ardisia japonica (Primulaceae), an evergreen clonal dwarf shrub

ABSTRACT Microsatellite markers were first developed for Ardisia japonica (Primulaceae), an evergreen clonal shrub that commonly grows in the understory of various types of temperate forests in East Asia. Of the 69 primer pairs designed based on genomic sequence data, 13 pairs showed clear microsatellite peaks and allelic polymorphisms with tetraploidy in ramets from two populations. Ramets were successfully assigned to multilocus lineages (genets) using microsatellite markers. The number of alleles per locus ranged from 3 to 8, and the observed heterozygosity ranged from 0.468 to 0.881. These will be useful for the assignment of genets and for investigating the genet-level population genetics and ecology of the understory clonal shrub.

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来源期刊

Journal of Forest Research 农林科学-林学

CiteScore

3.00

自引率

6.70%

发文量

审稿时长

3 months

期刊介绍： Journal of Forest Research publishes original articles, reviews, and short communications. It covers all aspects of forest research, both basic and applied, with the aim of encouraging international communication between scientists in different fields who share a common interest in forest science.