慢性淋巴细胞白血病患者中阿内洛病毒的鉴定和基因组特征

IF 2.1 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Tingwang Jiang, Qiang Liu, Fenying Lu, Teng He, Xiaoli Xiang, Zhicheng Zhang, Ying Sun
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引用次数: 0

摘要

意图宏基因组学显示,除了消化道,某些病毒也常见于人类血液中。为了探索和监测潜在的新型病毒,在中国常熟市第二人民医院采集了三份慢性淋巴细胞白血病患者的血清样本。材料和方法。我们使用无偏见的病毒宏基因组方法对三名慢性淋巴细胞白血病患者血清样本的病毒组进行了测序,随后使用MrBayes v3.2进行了最大似然系统发育分析。此外,在贝叶斯共有树中与anelloviruses的ORF1氨基酸序列进行了成对序列比较。后果已经鉴定了八种不同的含有完整ORF1基因的anellovirus的部分基因组。BLASTp结果表明,这些病毒与GenBank中最匹配的氨基酸序列一致性在56.22%至95.43%之间。基于ORF1的系统发育分析表明,7个序列属于α-力矩病毒属,1个序列属于γ-力矩病毒。结论。这项病毒学研究增加了我们对人类血清中anelloviruse多样性的了解,但还需要进一步研究来验证其与疾病的潜在相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and Genomic Characterization of Anelloviruses in Patients with Chronic Lymphocytic Leukemia
Purpose. Metagenomics has revealed that, in addition to the digestive tract, certain viruses are also commonly found in human blood. In order to explore and monitor potential novel viruses, three serum samples of patients with chronic lymphocytic leukemia were collected at the No. 2 People’s Hospital of Changshu City, China. Materials and Methods. We sequenced the virome of serum samples from three patients with chronic lymphocytic leukemia using an unbiased viral metagenomic approach and subsequently performed maximum likelihood phylogenetic analysis using MrBayes v3.2. In addition, pairwise sequence comparison was produced with ORF1 amino acid sequences of anelloviruses within Bayesian consensus tree. Results. Partial genomes of eight different anelloviruses containing the complete ORF1 gene have been identified. BLASTp results showed that the amino acid sequence identity of these viruses with the best match in GenBank was between 56.22% and 95.43%. Phylogenetic analysis based on ORF1 indicated that seven sequences belong to the genus Alphatorquevirus and one sequence belongs to the genus Gammatorquevirus. Conclusions. This virological investigation has increased our understanding of the diversity of anelloviruses in human serum, but further study is needed to verify its potential correlation with disease.
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
226
审稿时长
6 months
期刊介绍: The Journal of Clinical Pharmacy and Therapeutics provides a forum for clinicians, pharmacists and pharmacologists to explore and report on issues of common interest. Reports and commentaries on current issues in medical and pharmaceutical practice are encouraged. Papers on evidence-based clinical practice and multidisciplinary collaborative work are particularly welcome. Regular sections in the journal include: editorials, commentaries, reviews (including systematic overviews and meta-analyses), original research and reports, and book reviews. Its scope embraces all aspects of clinical drug development and therapeutics, including: Rational therapeutics Evidence-based practice Safety, cost-effectiveness and clinical efficacy of drugs Drug interactions Clinical impact of drug formulations Pharmacogenetics Personalised, stratified and translational medicine Clinical pharmacokinetics.
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