含藏红花粗提物纳米脂质体对C26结肠癌小鼠的抗肿瘤活性

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Sanaz Abbaszadegan, H. Hosseinzadeh, S. Alavizadeh, Marziyeh Moghri, A. Abbasi, M. Jaafari
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引用次数: 0

摘要

目的:藏红花(Crocus sativus L.花的脱水柱头)被认为是一种有效的抗癌和化学预防药物。本研究旨在设计含藏红花粗提物的聚乙二醇化纳米脂质体用于治疗癌症。材料与方法:采用脂质薄膜法制备含25 mg/ml藏红花水提物的聚乙二醇纳米脂质体。通过脂质体的大小、体外细胞毒性和体内对C26荷瘤小鼠的治疗作用来表征脂质体的性质。结果:随着胆固醇水平的升高,制剂的IC50值升高。脂质体表征说明了所选制剂的性质,z -平均尺寸:73.7±1.3 nm;Pdi: 0.103±0.035;zeta电位:-20.8 mV±3.7;%包封率:91±0.059,30% FBS中168 h后释放率:16.26±0.01。结论:选用的藏红花脂质体治疗荷瘤小鼠,首次以300 mg/kg的剂量静脉注射纳米藏红花脂质体,与阴性对照组相比,抗肿瘤性能显著提高,而与藏红花水提物相比,无显著差异。因此,为了获得人类使用的最佳配方,该配方值得进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-tumor activity of nanoliposomes containing crude extract of saffron in mice bearing C26 colon carcinoma
Objective(s): Saffron, the dehydrated stigma of the Crocus sativus L. flower, has been reputed as an effective anticancer and chemopreventive agent in cancer therapy. This study aimed to design PEGylated nanoliposomes containing crude extract of saffron for the treatment of cancer.Materials and Methods: Various PEGylated nanoliposomes containing 25 mg/ml aqueous extract of saffron were prepared using the thin lipid film method. The characterization of liposomes was indicated by their size, in vitro cytotoxicity, and in vivo therapeutic efficacy against C26 tumor-bearing mice. Results:  By increasing cholesterol levels, the IC50 values of the formulations increased. Liposome characterization illustrated the properties of formulation of choice, as follows: Z-average size: 73.7 ± 1.3 nm; PDI: 0.103 ± 0.035; zeta potential: -20.8 mV ± 3.7; % encapsulation: 91 ± 0.059, % release after 168 hours in 30% FBS: 16.26 ± 0.01.Conclusion: Treating tumor-bearing mice with the selected saffron liposomes indicated that, for the first time, the i.v. injection of nano-liposomal saffron at a dose of 300 mg/kg significantly increased the anti-tumor property compared to the negative control group, while no significant difference was observed compared with aqueous extract of saffron. Hence, to achieve an optimal formulation for human use, the formulation merits further study.
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
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审稿时长
12 weeks
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