炎性细胞因子在肱骨近端骨折间充质干细胞中的表达。

Q1 Biochemistry, Genetics and Molecular Biology
M. Viveiros, M. M. Viveiros, Márcia Guimarães Silva, R. Kaneno, Natália Porfírio Avelino, C. Rainho, S. Schellini
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引用次数: 1

摘要

背景间充质干细胞(MSCs)是治疗各种骨科疾病的绝佳选择。本研究旨在确定从肱骨近端骨折中获得的骨髓间充质干细胞是否可以作为获得人间充质细胞原代培养物的替代来源。使用Ficoll梯度分离方案收获MSC,并在体外培养至第三代。然后,使用干细胞特异性表面标记物通过流式细胞术对细胞进行免疫表型分析。还诱导细胞分化为成骨细胞和脂肪细胞,用于MSC的表征和确认。3、5或7天后,使用酶联免疫吸附试验(ELISA)评估培养物上清液中细胞因子白细胞介素(IL)-1β、IL-6、IL-8、IL-10、肿瘤坏死因子α(TNF-α)和干扰素γ(IFN-γ)的产生。结果免疫表型显示干细胞表面标志物CD73、CD90和CD105高表达,CD34、CD45、CD11b、CD19和人类白细胞抗原(HLA)-DR阴性或极低表达。骨髓来源的MSCs能够分化为成骨细胞和脂肪细胞。分泌细胞因子的定量显示,IL-8是产生最多的细胞因子,其次是IL-6和IL-10,数量相似,IL-1β水平较低。未检测到TNF-α和IFN-γ。结论肱骨近端骨折可作为骨髓间充质干细胞采集的替代来源。这些细胞的细胞因子产生与先前报道的骨折血肿的产生情况非常相似,可用于改善骨修复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of inflammatory cytokines in mesenchymal stem cells derived from proximal humerus fractures.
Background Mesenchymal stem cells (MSCs) are an excellent treatment option for a wide variety of orthopaedic conditions. This study aimed to establish if bone marrow MSCs obtained from proximal humerus fractures can be an alternative source for obtaining primary cultures of human MSCs. Methods Human bone marrow was obtained during osteosynthesis surgeries on closed proximal humerus fractures within 48 hours of injury. MSCs were harvested using the Ficoll gradient separation protocol and in vitro cultured until the third passage. Then, the cells were immunophenotyped by flow cytometry using stem cell specific surface markers. The cells were also induced to differentiate into osteoblasts and adipocytes for the characterization and confirmation of MSCs. The production of cytokines interleukin (IL)-1β, IL-6, IL-8, IL-10, tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) was assessed using enzyme-linked immunosorbent assay (ELISA) in the supernatant of the cultures after 3, 5 or 7 days. Results Immunophenotyping showed high expression of the stem cell surface markers CD73, CD90, and CD105 and negative or very low expression of CD34, CD45, CD11b, CD19, and human leukocyte antigen (HLA)-DR. The bone marrow derived MSCs were able to differentiate into osteoblasts and adipocytes. The quantification of secreted cytokines revealed that IL-8 was the most produced cytokine, followed by IL-6 and IL-10 at similar quantities and lower levels of IL-1β. TNF-α and IFN-γ were not detected. Conclusions Proximal humerus fractures can be an alternative source for the collection of bone marrow MSCs. The cytokine production of these cells is very similar to the production profile of fracture haematomas previously reported and may be used for improving bone repair.
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来源期刊
Stem cell investigation
Stem cell investigation Biochemistry, Genetics and Molecular Biology-Developmental Biology
CiteScore
5.80
自引率
0.00%
发文量
9
期刊介绍: The Stem Cell Investigation (SCI; Stem Cell Investig; Online ISSN: 2313-0792) is a free access, peer-reviewed online journal covering basic, translational, and clinical research on all aspects of stem cells. It publishes original research articles and reviews on embryonic stem cells, induced pluripotent stem cells, adult tissue-specific stem/progenitor cells, cancer stem like cells, stem cell niche, stem cell technology, stem cell based drug discovery, and regenerative medicine. Stem Cell Investigation is indexed in PubMed/PMC since April, 2016.
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