SARS-CoV-2刺突蛋白表位定位可区分疫苗接种者和感染者的抗体结合活性

IF 2 Q4 VIROLOGY
Nathaniel Felbinger, D. Trudil, L. Loomis, R. Ascione, G. Siragusa, S. Haba, Shruti Rastogi, Aidan Mucci, Mark Claycomb, Sebastian Snowberger, B. Luke, S. Francesconi, Shirley Tsang
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引用次数: 0

摘要

先前的研究试图利用肽微阵列在线性肽水平上表征个体对SARS-CoV-2病毒的抗体反应。这些研究有助于确定表位,这些表位有可能用于诊断测试,以识别受感染的个体。接种了美国最流行的两种疫苗(Moderna mRNA-1273或Pfizer BNT162b2 mRNA疫苗)的个体的免疫反应尚未确定。我们的目的是鉴定引起高IgG或IgA结合活性的SARS-CoV-2刺突蛋白的线性肽,并利用肽微阵列比较感染个体与接受两种剂量疫苗的个体的免疫反应性。我们的结果显示肽表位显著IgG结合最近感染的个体。其中一些肽位于受体结合域的可变区域附近,以及与SARS-CoV-2高传染性有关的刺突蛋白c端的保守区域。尽管总体抗体结合活性相似,但接种疫苗的个体对这些不同的标记物缺乏反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epitope mapping of SARS-CoV-2 spike protein differentiates the antibody binding activity in vaccinated and infected individuals
Previous studies have attempted to characterize the antibody response of individuals to the SARS-CoV-2 virus on a linear peptide level by utilizing peptide microarrays. These studies have helped to identify epitopes that have potential to be used for diagnostic tests to identify infected individuals. The immunological responses of individuals who have received the two most popular vaccines available in the US, the Moderna mRNA-1273 or the Pfizer BNT162b2 mRNA vaccines, have not been characterized. We aimed to identify linear peptides of the SARS-CoV-2 spike protein that elicited high IgG or IgA binding activity and to compare the immunoreactivity of infected individuals to those who received both doses of either vaccine by utilizing peptide microarrays. Our results revealed peptide epitopes of significant IgG binding among recently infected individuals. Some of these peptides are located near variable regions of the receptor binding domains as well as the conserved region in the c-terminal of the spike protein implicated in the high infectivity of SARS-CoV-2. Vaccinated individuals lacked a response to these distinct markers despite the overall antibody binding activity being similar.
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