多重PCR检测金枪鱼中沙门氏菌、大肠杆菌和单核增生李斯特菌

Kristin Natalia, M. Wahjudi, Tjie Kok
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引用次数: 0

摘要

渔业产品是一种具有不同发展水平的商品,包括添加食品添加剂。它们非常容易受到致斑微生物污染,因此可能导致食物中毒,并在消费者中引发疾病爆发。在这项研究中,对掺有沙门氏菌、大肠杆菌和李斯特菌的金枪鱼进行了细菌污染检测。使用多重PCR(mPCR)对这些致斑细菌进行检测,这是一种同时检测细菌靶DNA的现代技术。将检查结果与生化测试的灵敏度和时间效率进行了比较。基于所获得的结果,mPCR方法比生化测试的灵敏度高±1250倍,时间效率高±80倍。因此,mPCR方法可作为检测水产中细菌的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of Salmonella spp., Escherichia coli, and Listeria monocytogenes in Tuna by Multiplex PCR
Fishery products are types of commodity with various levels of development including the addition of food additives. They are very susceptible to patogenic microbial contamination hence they can cause food poisoning and cause disease outbreaks in consumers. In this study, tuna fish spiked with Salmonella spp., Escherichia coli, and Listeria monocytogenes bacteria is examined for the bacteria contamination. The examination of these patogenic bacteria is carried out using multiplex PCR (mPCR), a modern technique developed to detect target DNAs of bacteria simultaneously. The results of the examination are compared to biochemical test for sensitivity and time-efficiency. Based on the results obtained, mPCR method is ±1250-fold more sensitive and ±80-fold more time-efficient than the biochemical tests. Therefore, mPCR method could be recommended to be used as a tool for detection of bacteria in fishery products.
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