鸡肉中金黄色葡萄球菌的流行、表型-基因型耐药及生物膜的形成及其公共卫生危害

IF 0.3 4区农林科学 Q4 VETERINARY SCIENCES

Slovenian Veterinary Research Pub Date : 2023-02-26 DOI:10.26873/svr-1646-2022

A. Morshdy, A. Tharwat, A. Merwad, Nada A. M. Abdallah, Taisir Saber

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引用次数: 0

摘要

本研究的目的是确定金黄色葡萄球菌的流行率、耐药性以及从埃及Sharkia传统商店和超市的肉鸡中分离的金黄色葡萄球菌中产肠毒素和生物膜形成基因的存在。为此，从鸡胸和鸡腿各收集了200个新鲜生鸡肉棉签(各100个)。通过凝固酶检测对金黄色葡萄球菌进行标记，并对其进行表型和基因型分析。采用单链聚合酶链反应(PCR)对所选菌株进行肠毒素基因的检测。最后，使用96孔平底聚苯乙烯微滴板进行生物膜形成测试;此外，还对生物膜形成基因进行了研究。200个样品中19株金黄色葡萄球菌检测呈阳性(9.5%)，未检出万古霉素耐药菌株，也未检出环丙沙星金黄色葡萄球菌分离株，而所有分离株对链霉素、阿莫西林-克拉维酸和磺胺甲恶唑-甲氧苄啶均100%耐药。金黄色葡萄球菌多重抗生素耐药指数(MAR)范围为0.3 ~ 0.7，平均为0.4。耐甲氧西林金黄色葡萄球菌(MRSA)中携带mecA基因的比例为5%，携带blaZ基因的比例为5.5%(11/200)。所获得的分离株60%形成强生物膜，40%不形成生物膜，中等或弱生产分离株无结果。icaA基因为100%，而icaD基因为零。值得注意的是，强生物膜原菌株对sea和seb的检测仅为100%阳性。本研究指出，鸡肉中金黄色葡萄球菌耐多药分离株的较高流行率是由于处理不当、卫生设备不完善和后期微生物污染所致。这一发现强调了肉鸡肉作为耐抗生素金黄色葡萄球菌菌株和金黄色葡萄球菌生物膜形成库的重要性。

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查看原文本刊更多论文

PREVALENCE, PHENOTYPIC-GENOTYPIC RESISTANCE AND BIOFILM FORMATION OF Staphylococcus aureus IN CHICKEN MEAT WITH REFERENCE TO ITS PUBLIC HEALTH HAZARD

The purpose of this study was to determine the prevalence, antimicrobial resistance in S. aureus and presence of enterotoxigenic as well as biofilm-forming genes in S. aureus bacteria isolated from broiler meat from traditional shops and supermarkets in Sharkia, Egypt. For this determination, two hundred fresh raw chicken meat cotton swabs were collected from the breast and thighs (100 each). S. aureus was highlighted through a coagulase test, and then phenotypic and genotypic characterizations were studied. Uniplex PCR was used to identify the occurrence of enterotoxin genes in the selected isolates. Finally, they were subjected to a biofilm formation test using 96-well flat bottom polystyrene microtiter plates; besides, biofilm-forming genes were investigated. Nineteen isolates out of the 200 samples tested positive for S. aureus (9.5 percent), No Vancomycin resistance strains were obtained, nor did any ciprofloxacin isolates for S. aureus while, all isolates were 100 % resistant for streptomycin, amoxicillin- clavulanic acid and sulfamethoxazole-trimethoprim S. aureus isolates were found resistant to at least one antibiotic. The multiple antibiotic resistance (MAR) index of S. aureus isolates was ranged from 0.3 to 0.7 with an average of 0.4. In The mecicillin resistant S.aureus (MRSA) strains carried mecA gene with a percentage of 5%, while the blaZ gene was distributed with a percentage of 5.5%, 11/200). The obtained isolates gave 60% strong biofilm formation and 40% were non-formers, with nil results for moderate or weak production isolates. The icaA gene was 100%, in comparison to icaD which was zero. It should be noted that strong biofilm former strains were only 100% positive for sea and seb. This study pointed out the higher prevalence of MDR isolates of S. aureus isolates in chicken meat due to inadequate handling and insufficient sanitary equipment and post microbial contamination. This finding highlighted the importance of broiler meat as a reservoir for antimicrobial-resistant strains of S. aureus and biofilm-forming of Staphylococcus aureus.

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来源期刊

Slovenian Veterinary Research VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： SLOVENIAN VETERINARY RESEARCH (ISSN 1580-4003) publishes original articles, which report the results of original research in most areas of biomedicine. The journal also publishes review articles dealing with rapidly developing areas of biomedicine or which update understanding of classical fields of biomedicine, as well as case reports, shorter scientific contributions, letters to the editor, etc.; which have not been published or are under consideration for publication elsewhere. Only papers written in English can be considered.