Soudeh Ghafouri-Fard , Saede Atarbashi-Moghadam , Vahid Kholghi-Oskooei , Asghar Ashrafi Hafez , Mohammad Taheri
{"title":"涎腺恶性肿瘤中vdr相关lncrna的表达:一项初步研究","authors":"Soudeh Ghafouri-Fard , Saede Atarbashi-Moghadam , Vahid Kholghi-Oskooei , Asghar Ashrafi Hafez , Mohammad Taheri","doi":"10.1016/j.mgene.2021.100980","DOIUrl":null,"url":null,"abstract":"<div><p>Salivary gland tumors are complex neoplastic disorders with unidentified basis. Based on the importance of vitamin D receptor signaling in the pathogenesis of neoplastic conditions, we measured expression of some mRNA coding genes (<em>VDR</em>, <em>CYP24A1</em> and <em>CYP25B1</em>) and long non-coding RNAs (<em>SNHG16</em>, <em>SNHG6</em>, <em>LINC00346</em> and <em>LINC00511</em>) from this pathway in 42 paraffin-embedded blocks from 37 patients with different disorders originated from salivary gland. Total RNA was extracted from these samples using commercial kits and expression of mentioned genes was measured in these samples using quantitative real time PCR method. Expression of <em>LINC00511</em> was lower in tumor samples compared with pleomorphic adenoma (PA) samples (Expression ratio (ER) = 0.08, <em>P</em> value = 0.009). Yet, its expression was higher in PA samples compared with non-cancerous tissues adjacent to carcinoma samples (ER = 21.77, <em>P</em> = 0.009). In addition, its expression was lower in both adenoid cystic carcinoma and mucoepidermoid carcinoma samples compared with PA samples (ER = 0.11, <em>P</em> value = 0.046 and ER = 0.07, P value = 0.017, respectively). Expression of other genes was statistically similar between different lesions. Therefore, <em>LINC00511</em> might be used as a marker for separation of salivary gland carcinomas from PA samples.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"30 ","pages":"Article 100980"},"PeriodicalIF":0.8000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214540021001316/pdfft?md5=e8282337141fcee372e9076a651e5b02&pid=1-s2.0-S2214540021001316-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Expression of VDR-related lncRNAs in malignancies originated from salivary gland: A pilot study\",\"authors\":\"Soudeh Ghafouri-Fard , Saede Atarbashi-Moghadam , Vahid Kholghi-Oskooei , Asghar Ashrafi Hafez , Mohammad Taheri\",\"doi\":\"10.1016/j.mgene.2021.100980\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Salivary gland tumors are complex neoplastic disorders with unidentified basis. Based on the importance of vitamin D receptor signaling in the pathogenesis of neoplastic conditions, we measured expression of some mRNA coding genes (<em>VDR</em>, <em>CYP24A1</em> and <em>CYP25B1</em>) and long non-coding RNAs (<em>SNHG16</em>, <em>SNHG6</em>, <em>LINC00346</em> and <em>LINC00511</em>) from this pathway in 42 paraffin-embedded blocks from 37 patients with different disorders originated from salivary gland. Total RNA was extracted from these samples using commercial kits and expression of mentioned genes was measured in these samples using quantitative real time PCR method. Expression of <em>LINC00511</em> was lower in tumor samples compared with pleomorphic adenoma (PA) samples (Expression ratio (ER) = 0.08, <em>P</em> value = 0.009). Yet, its expression was higher in PA samples compared with non-cancerous tissues adjacent to carcinoma samples (ER = 21.77, <em>P</em> = 0.009). In addition, its expression was lower in both adenoid cystic carcinoma and mucoepidermoid carcinoma samples compared with PA samples (ER = 0.11, <em>P</em> value = 0.046 and ER = 0.07, P value = 0.017, respectively). Expression of other genes was statistically similar between different lesions. Therefore, <em>LINC00511</em> might be used as a marker for separation of salivary gland carcinomas from PA samples.</p></div>\",\"PeriodicalId\":38190,\"journal\":{\"name\":\"Meta Gene\",\"volume\":\"30 \",\"pages\":\"Article 100980\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2021-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214540021001316/pdfft?md5=e8282337141fcee372e9076a651e5b02&pid=1-s2.0-S2214540021001316-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Meta Gene\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214540021001316\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Meta Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214540021001316","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Expression of VDR-related lncRNAs in malignancies originated from salivary gland: A pilot study
Salivary gland tumors are complex neoplastic disorders with unidentified basis. Based on the importance of vitamin D receptor signaling in the pathogenesis of neoplastic conditions, we measured expression of some mRNA coding genes (VDR, CYP24A1 and CYP25B1) and long non-coding RNAs (SNHG16, SNHG6, LINC00346 and LINC00511) from this pathway in 42 paraffin-embedded blocks from 37 patients with different disorders originated from salivary gland. Total RNA was extracted from these samples using commercial kits and expression of mentioned genes was measured in these samples using quantitative real time PCR method. Expression of LINC00511 was lower in tumor samples compared with pleomorphic adenoma (PA) samples (Expression ratio (ER) = 0.08, P value = 0.009). Yet, its expression was higher in PA samples compared with non-cancerous tissues adjacent to carcinoma samples (ER = 21.77, P = 0.009). In addition, its expression was lower in both adenoid cystic carcinoma and mucoepidermoid carcinoma samples compared with PA samples (ER = 0.11, P value = 0.046 and ER = 0.07, P value = 0.017, respectively). Expression of other genes was statistically similar between different lesions. Therefore, LINC00511 might be used as a marker for separation of salivary gland carcinomas from PA samples.
Meta GeneBiochemistry, Genetics and Molecular Biology-Genetics
CiteScore
1.10
自引率
0.00%
发文量
20
期刊介绍：
Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.
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