暴露在锌和过氧化氢中的布拉蛙的即时反应

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-11-04 DOI:10.1111/pre.12471

A. Herbst, V. Ranawakage, T. Asaeda, H. Schubert

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引用次数: 1

摘要

在短时间暴露实验中，分析了锌（Zn）和过氧化氢（H2O2）对灰蝶的直接影响。暴露浓度分别为12.3、18.4和24.5μmol L−1 H2O2、12、60和120 mg L−1 Zn和12.3μmol L–1 H2O2 + 12 mg L−1 Zn，12.3μmol L−1 H2O2 + 60 mg L−1 Zn和18.4μmol L−1 H2O2 + 12 mg L−1锌。通过测定光合光系统II活性、叶绿素a和b以及类胡萝卜素含量、H2O2浓度以及抗坏血酸过氧化物酶、过氧化氢酶和愈创木酚过氧化物酶的抗氧化酶活性，分析了braunii的胁迫反应。锌的短期添加降低了braunii的色素含量。暴露于H2O2的braunii中的叶绿素a、b和类胡萝卜素含量与对照植物一样高。H2O2处理的braunii C.光合作用降低，短期添加Zn不会影响电子传输速率。在对照和暴露植物之间，braunii的H2O2浓度和抗氧化酶活性没有显著差异。描述了酶适应的趋势：H2O2诱导的应激反应的特征是抗氧化酶活性增加，而锌灭活了braunii中的过氧化氢酶。

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Immediate response of Chara braunii exposed to zinc and hydrogen peroxide

The immediate effect of zinc (Zn) and hydrogen peroxide (H2O2) in Chara braunii was analyzed in short‐time exposure experiments. The exposure concentrations were 12.3, 18.4, and 24.5 μmol L−1 H2O2, 12, 60, and 120 mg L−1 Zn, and 12.3 μmol L−1 H2O2 + 12 mg L−1 Zn, 12.3 μmol L−1 H2O2 + 60 mg L−1 Zn, and 18.4 μmol L−1 H2O2 + 12 mg L−1 Zn. The stress response of C. braunii was analyzed by measuring photosynthetic photosystem II activity, chlorophyll a and b and carotenoid contents, the H2O2 concentration, and antioxidant enzyme activities of ascorbic peroxidase, catalase, and guaiacol peroxidase. The short‐term addition of Zn reduced pigment contents in C. braunii. Chlorophyll a and b and carotenoid contents in H2O2‐exposed C. braunii were as high as in control plants. Photosynthesis was reduced in H2O2‐treated C. braunii and the short‐term addition of Zn did not affect the electron transport rate. H2O2 concentration and antioxidant enzyme activities in C. braunii were not significantly different between control and exposed plants. Trends of enzymatic adaptation were described: the H2O2‐induced stress response was characterized by increased antioxidant enzyme activities, whereas Zn inactivated catalase in C. braunii.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.