HPLC-DAD法测定花卉和食品补充剂中的类胡萝卜素

IF 0.9 Q4 CHEMISTRY, MULTIDISCIPLINARY
P. Pavelková, A. Krmela, V. Schulzova
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引用次数: 7

摘要

摘要选择万寿菊(万寿菊和金盏菊)进行分析,因为它们是捷克市场上食品补充剂生产中最常用的叶黄素及其异构体玉米黄质来源。比较了直接提取法和碱水解提取法对游离类胡萝卜素和结合类胡萝卜素的检测效果。类胡萝卜素分离采用C18和C30色谱柱。建立了一种测定胶囊形式的食品补充剂中类胡萝卜素含量的新方法。所有基质采用高效液相色谱二极管阵列检测(HPLC-DAD)进行分析。人们发现,万寿菊花和食品补充剂都需要碱性水解才能将叶黄素从酯键释放到脂肪酸中。在金盏菊干物质中检测到叶黄素浓度为807 ~ 1472 mg·kg−1。叶片万寿菊的干物质含量为5906 ~ 8677 mg·kg−1,是较好的叶黄素来源。商业食品补充剂(叶黄素复合物Premium和Occutein brilliant)中叶黄素和玉米黄素的含量与申报量一致。HPLC-DAD方法的线性范围为0.1 ~ 20 μg·mL−1,万寿菊中叶黄素的定量限为1.7 mg·kg−1,食品补充剂中叶黄素的定量限为200 mg·kg−1。叶黄素在所有测试基质中的重复性为2.3%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of carotenoids in flowers and food supplements by HPLC-DAD
Abstract Marigold flowers (Tagetes patula and Calendula officinalis) were chosen for analysis because they are the most often used source of lutein and its isomer zeaxanthin for the production of food supplements on the Czech market. Direct extraction and extraction with alkaline hydrolysis were compared to detect free or bound carotenoids. For carotenoid separation, C18 and C30 columns were used. A new method for determination of carotenoid content in food supplements in form of capsules has been developed and validated. All matrices were analysed by high-performance liquid chromatography with diode array detection (HPLC-DAD). It has been found that alkaline hydrolysis is required for both Marigold flowers and food supplements to release lutein from ester bonds to fatty acids. In Calendula officinalis lutein in the concentration of 807—1472 mg·kg−1 of dry matter was detected. Tagetes patula has been identified as a better lutein source with the content of 5906—8677 mg·kg−1 of dry matter. It has been found that the content of lutein and zeaxanthin in commercial food supplements (Lutein Complex Premium and Occutein Brillant) is consistent with the declared quantity. Linearity of the HPLC-DAD method ranged from 0.1—20 μg·mL−1 with the limit of quantification (LOQ) of 1.7 mg·kg−1 for lutein in Marigold flowers and 200 mg·kg−1 in food supplements. Repeatability was 2.3 % for lutein in all tested matrices.
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来源期刊
Acta Chimica Slovaca
Acta Chimica Slovaca CHEMISTRY, MULTIDISCIPLINARY-
自引率
12.50%
发文量
11
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