两种禽流感疫苗对家禽血清转化率的比较评价

Q3 Veterinary

American Journal of Animal and Veterinary Sciences Pub Date : 2022-03-01 DOI:10.3844/ajavsp.2022.211.218

Belan A. Astemirov, S. Mamadaliyev, Yuliya V. Perfiliyeva, Marina A. Kopochenya

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引用次数: 0

摘要

: 2020年8月至12月，H5禽流感的新暴发在哈萨克斯坦造成了另一次严重的动物卫生紧急情况，导致50多万只鸡死亡或被扑杀。这次疫情再次引起人们对制定预防这种再次出现的感染的战略的兴趣。在这项研究中，我们评估了两种H5疫苗后家禽的血清转化水平。无论年龄、生产力和疫苗接种方案如何，超级尼克层(共368头)分别接种了一剂灭活的全H5疫苗或杆状病毒衍生的H5疫苗。我们通过血凝抑制(HI)和酶联免疫吸附(ELISA)检测来评估血清转化。采用实时聚合酶链反应(real-time PCR)技术检测本地区的流感病毒(AIV)。对368份血清样本的分析表明，在接种后30,60和120天(DPV)，通过ELISA和HI评估，与杆状病毒衍生疫苗相比，灭活疫苗提供了显着更高的体液免疫应答。因此，我们的研究表明，在农场条件下，与杆状病毒衍生的H5疫苗相比，经典灭活禽流感(AIV)疫苗可诱导更高的H5N1病毒血清转化水平，这预示着对田间感染的保护作用更好。

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Comparative Assessment of Seroconversion in Poultry Vaccinated with Two Avian Influenza Vaccines

: From August to December 2020, a new outbreak of H5 avian influenza caused another serious animal health emergency in Kazakhstan, leading to the deaths or culling of more than 500 000 chickens. This outbreak renewed interest in developing prevention strategies for this re-emerging infection. In this study, we evaluated poultry seroconversion levels after two H5 vaccines. Regardless of age, productivity, and scheme of vaccination Super Nick layers (a total of 368 heads) received a single dose of an inactivated whole H5 vaccine or a baculovirus-derived H5 vaccine, respectively. We assessed the seroconversion by Hemagglutination Inhibition (HI) and Enzyme-Linked Immunosorbent (ELISA) assays. Cloacal and tracheal swabs were tested for Influenza A Virus (AIV) by real-time PCR to monitor the field AIV circulation. The analysis of 368 serum samples showed that the inactivated vaccine provided a significantly higher humoral immune response when compared to the baculovirus-derived vaccine as evaluated by both ELISA and HI in 30, 60, and 120 Days Post-Vaccination (DPV). Thus, our study demonstrates that under farm conditions classical Inactivated Avian Influenza (AIV) vaccine induces a higher seroconversion level against the H5N1 virus predicting better protection against field infection, than a baculovirus-derived H5 vaccine.

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来源期刊

American Journal of Animal and Veterinary Sciences Veterinary-Veterinary (all)

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： American Journal of Animal and Veterinary Sciences, a quarterly, peer reviewed publication and is dedicated for publication of research articles in the field of biology of animals and with the scientific understanding of how animals work: from the physiology and biochemistry of tissues and major organ systems down to the structure and function of bio molecules and cells; particular emphasis would given to the studies of growth, reproduction, nutrition and lactation of farm and companion animals and how these processes may be optimized to improve animal re- productivity, health and welfare. Articles in support areas, such as genetics, soils, agricultural economics and marketing, legal aspects and the environment also are encouraged. AJAVS is an important source of researcher to study articles on protection of animal production practices, herd health and monitoring the spread of disease and prevention in both domestic and wild animals.