一个简单和经济有效的体重下降模型诱导挫伤脊髓损伤:功能和组织学结果

IF 0.4 Q4 NEUROSCIENCES

Archives of Neuroscience Pub Date : 2021-12-19 DOI:10.5812/ans.118775

S. Hashemizadeh, Saereh Hoseindoost, Khalil Pestei, M. Hadjighassem

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引用次数: 3

摘要

背景:动物脊髓损伤(SCI)模型为了解创伤性脊髓损伤的相关机制和评估实验性治疗干预措施的有效性提供了更好的方法。目的:本研究的目的是开发一种具有成本效益的改良Allen's装置来诱导挫伤脊髓损伤。方法:采用定制的减重模型，将10 g重量从25 mm高度投放到暴露的脊髓上，造成成年雄性Wistar大鼠脊髓挫伤。观察28天的运动和感觉功能。此外，在脊髓损伤后1周和28天评估组织病理学变化。结果:所有大鼠脊髓损伤后48 h均出现后肢瘫痪和损伤后神经性疼痛。观察到与以往报道的挫伤模型相似的组织学变化。结论:根据我们的研究结果，损伤后28天单个大鼠的BBB评分几乎没有变化。我们定制的诱导脊髓损伤装置是一种简单而廉价的方法，可以替代通常用于诱导脊髓损伤的高度复杂的挫伤装置。

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A Simple and Cost-Effective Weight Drop Model to Induce Contusive Spinal Cord Injury: Functional and Histological Outcomes

Background: Animal spinal cord injury (SCI) models have provided a better perception of the mechanisms related to traumatic SCI and evaluation of the effectiveness of experimental therapeutic interventions. Objectives: The aim of this study is to develop a cost-effective modified Allen's device to induce contusive spinal cord injury. Methods: Adult male Wistar rats were subjected to contusive spinal cord injury using a customized weight drop model through 10-g weights delivered from a 25-mm height onto an exposed spinal cord. Locomotor and sensory function during 28 days were assessed. Moreover, histopathological changes were assessed at one week and 28 days post SCI. Results: All the SCI rats showed hind limb paralysis up to 48 h post SCI and neuropathic pain after injury. Histological changes similar to the previous reports for contusion model were observed. Conclusions: According to our findings, little variability was observed in the BBB score of individual rats at 28 days after injury. Our customized device to induce spinal cord injury is a simple and inexpensive alternative method to the highly sophisticated contusion device commonly used to induce SCI.

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Archives of Neuroscience NEUROSCIENCES-

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期刊介绍： Archives of neuroscience is a clinical and basic journal which is informative to all practitioners like Neurosurgeons, Neurologists, Psychiatrists, Neuroscientists. It is the official journal of Brain and Spinal Injury Research Center. The Major theme of this journal is to follow the path of scientific collaboration, spontaneity, and goodwill for the future, by providing up-to-date knowledge for the readers. The journal aims at covering different fields, as the name implies, ranging from research in basic and clinical sciences to core topics such as patient care, education, procuring and correct utilization of resources and bringing to limelight the cherished goals of the institute in providing a standard care for the physically disabled patients. This quarterly journal offers a venue for our researchers and scientists to vent their innovative and constructive research works. The scope of the journal is as far wide as the universe as being declared by the name of the journal, but our aim is to pursue our sacred goals in providing a panacea for the intractable ailments, which leave a psychological element in the daily life of such patients. This authoritative clinical and basic journal was founded by Professor Madjid Samii in 2012.