人ADAR1的Zα结构域与CG重复DNA双链优先结合的NMR研究

IF 0.4 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of the Korean magnetic resonance society Pub Date : 2017-09-20 DOI:10.6564/JKMRS.2017.21.3.090

Ae-Ree Lee, Seo-Ree Choi, Yeo-Jin Seo, Joon-Hwa Lee

{"title":"人ADAR1的Zα结构域与CG重复DNA双链优先结合的NMR研究","authors":"Ae-Ree Lee, Seo-Ree Choi, Yeo-Jin Seo, Joon-Hwa Lee","doi":"10.6564/JKMRS.2017.21.3.090","DOIUrl":null,"url":null,"abstract":"The Z-DNA domain of human ADAR1 (Zα ADAR1 ) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region. 9 However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of Zα ADAR1 . Thus, we have performed NMR experiments of Zα ADAR1 to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that Zα ADAR1 preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.","PeriodicalId":17414,"journal":{"name":"Journal of the Korean magnetic resonance society","volume":"21 1","pages":"90-95"},"PeriodicalIF":0.4000,"publicationDate":"2017-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NMR Study on the Preferential Binding of the Zα Domain of Human ADAR1 to CG-repeat DNA Duplex\",\"authors\":\"Ae-Ree Lee, Seo-Ree Choi, Yeo-Jin Seo, Joon-Hwa Lee\",\"doi\":\"10.6564/JKMRS.2017.21.3.090\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Z-DNA domain of human ADAR1 (Zα ADAR1 ) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region. 9 However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of Zα ADAR1 . Thus, we have performed NMR experiments of Zα ADAR1 to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that Zα ADAR1 preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.\",\"PeriodicalId\":17414,\"journal\":{\"name\":\"Journal of the Korean magnetic resonance society\",\"volume\":\"21 1\",\"pages\":\"90-95\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2017-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Korean magnetic resonance society\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.6564/JKMRS.2017.21.3.090\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Korean magnetic resonance society","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.6564/JKMRS.2017.21.3.090","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

人类ADAR1的Z-DNA结构域（ZαADAR1）通过优先结合CG重复片段和破坏邻近富含AT的区域的稳定来产生B-Z连接DNA。9然而，这项研究无法回答富含AT的区域中有多少碱基对因ZαADAR1的结合而不稳定的问题。因此，我们对含有8碱基对（8-bp）CG重复片段和12bp AT富集区的较长DNA双链体进行了ZαADAR1的NMR实验。这项研究表明，在长DNA中，ZαADAR1优先与CG重复片段结合，而不是与富含AT的区域结合，然后使相邻富含AT区域中的至少6个碱基对不稳定，以实现CG重复片段的有效B-Z过渡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

NMR Study on the Preferential Binding of the Zα Domain of Human ADAR1 to CG-repeat DNA Duplex

The Z-DNA domain of human ADAR1 (Zα ADAR1 ) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region. 9 However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of Zα ADAR1 . Thus, we have performed NMR experiments of Zα ADAR1 to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that Zα ADAR1 preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of the Korean magnetic resonance society BIOCHEMICAL RESEARCH METHODS-

自引率

66.70%

发文量