中国荷斯坦奶牛热休克蛋白70 - 1基因与细胞耐热性相关的单核苷酸多态性

Q4 Biochemistry, Genetics and Molecular Biology

Animal Gene Pub Date : 2021-06-01 DOI:10.1016/j.angen.2021.200114

Tarig Badri , Murtada Alsiddig , Li Lian , Yafei Cai , Genlin Wang

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引用次数: 2

摘要

热休克蛋白70kda -A1 (HSP70A1A)的5 '调控区和翻译区多态性被认为是奶牛对热应激的细胞保护和耐热性的相关基因。本研究旨在确定中国荷斯坦牛诱导热休克蛋白70-1基因的遗传变异及其与耐热性的关系。利用直接测序技术，在149头多产奶牛中新鉴定出3个单核苷酸多态性(snp)。荧光素酶报告基因测定方法显示，GG基因型在g -133(突变型)中比CC(野生型)增加了启动子活性。我们使用DNAStar Protean软件检测HSP70-1蛋白的亲水性和二级结构。结果显示，编码区的SNP (g. 462 g /T)改变了154 Gln→His的氨基酸序列。这些结果表明，HSP70-1基因的遗传多态性可能影响植物的耐热性，可以作为热调节的分子标记。因此，本研究可能有助于这些标记在中国荷斯坦耐热性遗传选择中发挥作用。

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Single nucleotide polymorphisms in HSP70–1 gene associated with cellular heat tolerance in Chinese Holstein cows

Polymorphisms in the 5′ regulatory and translated regions of a heat shock protein 70 kDa protein –A1 (HSP70A1A) are observed as the responsible for cellular protection against heat stress and associated with heat tolerance in dairy cows. The present study was conducted to determine the genetic variation in the inducible heat shock protein 70–1 gene and its association with heat resistance in Chinese Holstein cattle. Three single nucleotide polymorphisms (SNPs) were newly identified in 149 multiparous lactating cows, using direct sequencing. Luciferase reporter assay method was conducted and showed that GG genotype in g.-133 (mutant) increased the promoter activity than did CC (wildtype). We performed DNAStar Protean Software to detect the hydrophilicity and secondary structure of the HSP70–1 protein. The results revealed the SNP in the coding region (g. 462 G/T) was found to bring changes to the amino acid sequence at 154 Gln → His. These results suggested that the genetic polymorphisms in the HSP70–1 gene may influence the heat resistance, and it can be used as a molecular marker in thermoregulation. Therefore, this study may prove beneficial of these markers in genetic selections for heat tolerance in Chinese Holstein.

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来源期刊

Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.