从线粒体DNA序列推断西开普省和北开普省的Kazimierzus Plisko，2006（Clitellata，Kazimierszidae）的分子系统发育

IF 0.8 4区农林科学 Q3 ENTOMOLOGY

African Invertebrates Pub Date : 2020-07-10 DOI:10.3897/afrinvertebr.61.53380

T. Nxele, J. D. Plisko, T. Mwabvu, O. Zishiri

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引用次数: 2

摘要

使用形态学对蚯蚓进行物种鉴定可能具有挑战性，而且不确定，因为许多特征的同源性很高。分子DNA技术的使用，如mtDNA和核DNA中保守区的使用，揭示了几种蚯蚓物种的系统发育背景。目前的研究利用细胞色素c氧化酶亚基I（COI）线粒体标记重建了2006年南非西开普省和北开普省Kazimierzus Plisko物种的系统发育。系统发育重建使用贝叶斯推断和最大似然进行。两种造树方法都坚持大多数分类群的单系性。结果表明，该物种分为两个分支，并验证了目前已知的11个Kazimierzus物种（K.circulatus（Plisko，1998）、K.francicus（Pickford，1975）、K.guntheri（Pick福德，1975），K.hamerae（Plisco，1998），K.kleinoodi Nxele&Plisko（2017），K.nietvoorbiji Nxele＆Plisko，K.pearsonianus（皮克福德，1975），K.phumlani-Nxele&Plisko，2017，K.sophieae（Plisko），2002）。隐性多样性在具有遗传多样性的K.occidualis中很明显非洲无脊椎动物61（2）：83–92（2020）doi:10.3897/AfrInvertebr.61.53380http://africaninvertebrates.pensoft.net版权所有Thembeka Clara Nxele等人。这是一篇根据知识共享署名许可证（CC BY 4.0）条款分发的开放获取文章，该许可证允许在任何媒体上不受限制地使用、分发和复制，前提是原始作者和来源可信。研究文章Thembeka Clara Nxele等人/非洲无脊椎动物61（2）：83-92（2020）84种群数量超过12%。Kazimierzus franciscus和K.ljungstroemi（皮克福德，1975）具有较低的遗传变异性，表明亲缘关系密切或可能是同种。一组来自Clanwilliam的标本在形态上与锦葵科相同，但在基因上不同，可能属于未描述的物种。本研究证明了蚯蚓综合分类学的重要性，以便提供可靠的分类学和生物地理学数据。

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Molecular phylogeny of Kazimierzus Plisko, 2006 (Clitellata, Kazimierzidae) from the Western and Northern Cape Province inferred from mitochondrial DNA sequences

Species identification of earthworms using morphology can be challenging and inconclusive as homoplasy in many characters is high. The use of molecular DNA technology, such as the use of conserved regions in mtDNA and nuclear DNA has unravelled the phylogenetic background of several earthworm species. The current study utilised the cytochrome c oxidase subunit I (COI) mitochondrial marker to reconstruct the phylogeny of Kazimierzus Plisko, 2006 species from the Western and Northern Cape provinces of South Africa. Phylogenetic reconstructions were implemented using Bayesian Inference, as well as Maximum Likelihood. Both tree building methods adhered to the monophyly of the majority of the taxa. Results showed that species fell into two clades and validated eleven currently known Kazimierzus species (K. circulatus (Plisko, 1998), K. franciscus (Pickford, 1975), K. guntheri (Pickford, 1975), K. hamerae (Plisko, 1998), K. kleinoodi Nxele & Plisko, 2017, K. nietvoorbiji Nxele & Plisko, 2017, K. nieuwoudtvillensis Nxele & Plisko, 2017, K. occidualis (Plisko, 1998), K. pearsonianus (Pickford, 1975), K. phumlani Nxele & Plisko, 2017, K. sophieae (Plisko, 2002)). Cryptic diversity is evident in K. occidualis with genetic diverAfrican Invertebrates 61(2): 83–92 (2020) doi: 10.3897/AfrInvertebr.61.53380 http://africaninvertebrates.pensoft.net Copyright Thembeka Clara Nxele et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. RESEARCH ARTICLE Thembeka Clara Nxele et al. / African Invertebrates 61(2): 83–92 (2020) 84 gence greater than 12% amongst populations. Kazimierzus franciscus and K. ljungstroemi (Pickford, 1975) have a low genetic variability suggesting close relatedness or probably conspecificity. A group of specimens from Clanwilliam are morphologically identical to K. sophieae, but are genetically distinct and may belong to undescribed species. This study demonstrates the importance of integrative taxonomy in earthworms in order to present reliable taxonomic and biogeographic data.

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来源期刊

African Invertebrates 生物-动物学

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： African Invertebrates is an international peer-reviewed, open-access journal that focuses primarily on the taxonomy, systematics, biogeography, and palaeontology of Afrotropical invertebrates, whether terrestrial, freshwater or marine. Aspects concerning biology, ecology, and conservation may also be considered where these relate to the primary focus areas. Papers dealing solely with biology, ecology, physiology, pests and pest control should be submitted elsewhere.