铁积累通过破坏Wnt信号通路诱导骨质疏松

Q4 Medicine

中华内分泌代谢杂志 Pub Date : 2019-12-25 DOI:10.3760/CMA.J.ISSN.1000-6699.2019.12.012

Guangfei Li, Hui Zhang, Aifei Wang, Zihou Cao, G. Yan

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引用次数: 0

摘要

取6月龄野生型小鼠(WT)和6月龄hepcidin敲除(KO)小鼠(铁积累模型)股骨标本进行Micro-CT检查。Western blot和免疫共沉淀法检测Wnt信号通路相关参数的变化。与野生型小鼠相比，Hepcidin-KO小鼠的骨量明显减少，β-catenin与FOXO3a的结合增加，骨组织中β-catenin与TCF4/TCF7L2的结合减少，β-catenin、TCF4/TCF7L2和FOXO3a的表达无明显变化。这些结果提示，铁积累可能通过干扰典型的Wnt/β-catenin信号通路影响骨形成，最终导致骨质疏松。关键词:铁富集;骨质疏松症;Wnt信号通路

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Iron accumulation induces osteoporosis by disrupting Wnt signaling pathway

The specimens of femur from wild-type mice(WT) of 6 months and Hepcidin-knockout(KO) mice of 6 months(iron accumulation model) were obtained for Micro-CT examination. Western blot and co-immunoprecipitation were used to detect the changes of related parameters in Wnt signaling pathway. Compared with wild-type mice, the bone mass in Hepcidin-KO mice was significantly decreased, the binding of β-catenin to FOXO3a increased, and binding of β-catenin to TCF4/TCF7L2 decreased in bone tissue, without significant changes in the expression of β-catenin, TCF4/TCF7L2, and FOXO3a. These results suggest that iron accumulation may affect bone formation through interfering with canonical Wnt/β-catenin signaling pathway, finally leading to osteoporosis. Key words: Iron accumulation; Osteoporosis; Wnt signaling pathway

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来源期刊

中华内分泌代谢杂志 Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

0.60

自引率

0.00%

发文量

7243

期刊介绍： The Chinese Journal of Endocrinology and Metabolism was founded in July 1985. It is a senior academic journal in the field of endocrinology and metabolism sponsored by the Chinese Medical Association. The journal aims to be the "Chinese broadcaster of new knowledge on endocrinology and metabolism worldwide". It reports leading scientific research results and clinical diagnosis and treatment experience in endocrinology and metabolism and related fields, as well as basic theoretical research that has a guiding role in endocrinology and metabolism clinics and is closely integrated with clinics. The journal is a core journal of Chinese science and technology (a statistical source journal of Chinese science and technology papers), and is included in Chinese and foreign statistical source journal databases such as the Chinese Science and Technology Papers and Citation Database, Chemical Abstracts, and Scopus.