公鸡精液运动参数LIN、STR、WOB、ALH和BCF对氧化应激的反应

C. Pilane, M. H. Mapeka
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The semen motility, kinematic and viability parameters were then \ndetermined using the CASA system while the viability was determined using the \nSYBR-14/PI staining. The Pearson’s correlation coefficient was determined to \ntest the relationships between the levels of induced oxidative stress, period \nof exposure to oxidative stress inducing agent and the motility plus kinematic \nparameters. Our data revealed that in raw cockerel semen, there was high and \npositive correlations between total motility (TM), progressive motility (PM), \nrapid (RAP), curvilinear velocity (VCL), straight line velocity (VSL) and \naverage path velocity (VAP) while the kinematic parameters LIN, STR, WOB, ALH \nand BCF had low or negative correlations with them. Furthermore, TM, PM, RAP, \nVCL and VSL remained highly and positively correlated with the induced \noxidative stress and also, linearity (LIN), straightness (STR), wobble (WOB), \namplitude of lateral head displacement (ALH) and beat cross frequency (BCF) \nremained negatively correlated with the induced oxidative stress, after 3 hrs. \nAfter 24 hrs, TM, PM, RAP, VCL, VSL, VAP and ALH, became negatively correlated \nwith the induced oxidative stress while LIN, STR, WOB and BCF became positively \ncorrelated with the induced oxidative stress. Conversely, when the H2O2 \nconcentration used was correlated with motility and kinematic parameters over \ntime, TM, PM, RAP, VCL, VSL, VAP became negatively correlated with oxidative \nstress while LIN, STR, WOB, ALH and BCF show negative or low correlations with \nthe induced oxidative stress. This data indicates that LIN, STR, WOB, BCF and \nto some extend ALH, reveal the least correlations with the induced oxidative \nstress under persistent oxidative stress conditions in cockerel semen. In \nconclusion, cockerel semen, like buck semen, does not easily succumb to \noxidative stress since the raw semen correlations of CASA analysed parameters \nare comparable to these observed after 3 hrs of H2O2 \ntreatment. In addition, the oxidative stress levels tolerated by cockerel semen \nshould not 5 &microM H2O2 \noxidative stress levels. 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引用次数: 1

摘要

计算机辅助精子类分析仪(CASA)对冷冻保存后的鸟类精液进行分析表明,它们的精液活力和活力参数受到损害,部分原因是氧化应激。为了模拟这些观察结果,我们用氧化应激诱导剂,即过氧化氢(H2O2)处理公鸡精液,并随着时间的推移监测其运动性、运动学和活力参数。简单地说,选择5只健康的、可生育的南非文达小公鸡,用腹部按摩技术收集它们的精液。然后将精液用浓度分别为0 μ m、5 μ m、50 μ m和200 μ m的H2O2处理0、3、16和24小时。采用CASA系统测定精子活力、运动学和活力参数,SYBR-14/PI染色测定精子活力。测定Pearson相关系数,检验诱导氧化应激水平、氧化应激诱导剂暴露时间与运动和运动学参数之间的关系。结果表明,公鸡精液的总运动力(TM)、前进运动力(PM)、快速运动速度(RAP)、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径运动速度(VAP)呈高度正相关,而运动学参数LIN、STR、WOB、ALH和BCF与它们呈低相关或负相关。3 h后,TM、PM、RAP、VCL和VSL与诱导氧化应激呈高度正相关,线性度(LIN)、直线度(STR)、摆动度(WOB)、侧头位移幅度(ALH)和搏动交叉频率(BCF)与诱导氧化应激呈负相关。24 h后,TM、PM、RAP、VCL、VSL、VAP和ALH与诱导的氧化应激呈负相关,LIN、STR、WOB和BCF与诱导的氧化应激呈正相关。相反,当使用的H2O2浓度随时间与运动和运动学参数相关时,TM、PM、RAP、VCL、VSL、VAP与氧化应激呈负相关,而LIN、STR、WOB、ALH和BCF与诱导的氧化应激呈负相关或低相关。这一数据表明,在持续氧化应激条件下,公鸡精液中LIN、STR、WOB、BCF以及一定程度上的ALH与诱导氧化应激的相关性最小。总之,公鸡精液和雄鹿精液一样,不容易受到氧化应激的影响,因为CASA分析的原始精液参数的相关性与经过3小时H2O2处理后观察到的结果相当。此外,公鸡精液耐受的氧化应激水平不应超过5微米H2O2氧化应激水平。最后,在公鸡精液中,LIN、STR、WOB、BCF和ALH与诱导的氧化应激缺乏相关性,可用于显示难以忍受的低温保存条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Response of Cockerel Semen Kinematic Parameters LIN, STR, WOB, ALH and BCF to Induced Oxidative Stress
Computer-assisted sperm class analyser (CASA) analysis of avian semen following cryopreservation indicates that their semen motility and viability parameters become compromised, due in part to oxidative stress. To mimic these observations we have treated cockerel semen with an oxidative stress inducing agent, namely hydrogen peroxide (H2O2) and monitored the motility, kinematic and viability parameters over time. Briefly, five healthy and fertile South African Venda cockerels were selected and their semen was collected using the abdominal massage technique. The semen was then treated with H2O2 at 0 µM, 5 µM, 50 µM and 200 µM concentrations for 0, 3, 16 and 24 hrs. The semen motility, kinematic and viability parameters were then determined using the CASA system while the viability was determined using the SYBR-14/PI staining. The Pearson’s correlation coefficient was determined to test the relationships between the levels of induced oxidative stress, period of exposure to oxidative stress inducing agent and the motility plus kinematic parameters. Our data revealed that in raw cockerel semen, there was high and positive correlations between total motility (TM), progressive motility (PM), rapid (RAP), curvilinear velocity (VCL), straight line velocity (VSL) and average path velocity (VAP) while the kinematic parameters LIN, STR, WOB, ALH and BCF had low or negative correlations with them. Furthermore, TM, PM, RAP, VCL and VSL remained highly and positively correlated with the induced oxidative stress and also, linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) remained negatively correlated with the induced oxidative stress, after 3 hrs. After 24 hrs, TM, PM, RAP, VCL, VSL, VAP and ALH, became negatively correlated with the induced oxidative stress while LIN, STR, WOB and BCF became positively correlated with the induced oxidative stress. Conversely, when the H2O2 concentration used was correlated with motility and kinematic parameters over time, TM, PM, RAP, VCL, VSL, VAP became negatively correlated with oxidative stress while LIN, STR, WOB, ALH and BCF show negative or low correlations with the induced oxidative stress. This data indicates that LIN, STR, WOB, BCF and to some extend ALH, reveal the least correlations with the induced oxidative stress under persistent oxidative stress conditions in cockerel semen. In conclusion, cockerel semen, like buck semen, does not easily succumb to oxidative stress since the raw semen correlations of CASA analysed parameters are comparable to these observed after 3 hrs of H2O2 treatment. In addition, the oxidative stress levels tolerated by cockerel semen should not 5 µM H2O2 oxidative stress levels. Lastly, lack of correlation between LIN, STR, WOB, BCF and ALH and induced oxidative stress can be used in cockerel semen to show intolerable cryopreservation conditions.
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