{"title":"对鱼的性腺进行固定的显微镜标本检查的程序","authors":"Q. Dinh, T. H. Nguyen","doi":"10.12982/vis.2022.049","DOIUrl":null,"url":null,"abstract":"This study aims to provide a procedure for performing microscopy in fish gonads. Specimens used in this study were the ovaries and testes of fish collected in the Mekong Delta from April to July 2022. Organs at five different stages for each sex (ovary diameter from 1.16 cm in stage I to 4.04 cm in stage V; testis diameter from 0.42 in stage I to 2.39 in stage V) were studied in this procedure. The results showed that both ovaries and testes needed to be fixed at 4% formol for 24 h and under running water for eight hours. Ovaries and testes were then immersed in 500 ethanol for 24 h to initiate dehydration. Results showed that the time for dehydration and paraffin infiltration was longer in the ovary (26 to 55 h across stages) than in the testis (25 to 26 h across stages). The mean staining time of the ovary was 1.5 min (stage I) to 3.5 min (stage V) in Hematoxylin and 50 s (stage I) to 140 s (stage V) in Eosin. Whereas the staining time of testis was shorter, 1 min (stage I) to 3 min (stage V) in Hematoxylin and 30 s (stage I) to 90 s (stage V) in Eosin. This study provides a reference for further studies on the histological structure of fish gonads. These results could be applied to fish species inside and outside the Mekong Delta.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Procedure for performing a fixed microscopic specimen of the gonads of fish\",\"authors\":\"Q. Dinh, T. H. Nguyen\",\"doi\":\"10.12982/vis.2022.049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aims to provide a procedure for performing microscopy in fish gonads. Specimens used in this study were the ovaries and testes of fish collected in the Mekong Delta from April to July 2022. Organs at five different stages for each sex (ovary diameter from 1.16 cm in stage I to 4.04 cm in stage V; testis diameter from 0.42 in stage I to 2.39 in stage V) were studied in this procedure. The results showed that both ovaries and testes needed to be fixed at 4% formol for 24 h and under running water for eight hours. Ovaries and testes were then immersed in 500 ethanol for 24 h to initiate dehydration. Results showed that the time for dehydration and paraffin infiltration was longer in the ovary (26 to 55 h across stages) than in the testis (25 to 26 h across stages). The mean staining time of the ovary was 1.5 min (stage I) to 3.5 min (stage V) in Hematoxylin and 50 s (stage I) to 140 s (stage V) in Eosin. Whereas the staining time of testis was shorter, 1 min (stage I) to 3 min (stage V) in Hematoxylin and 30 s (stage I) to 90 s (stage V) in Eosin. This study provides a reference for further studies on the histological structure of fish gonads. These results could be applied to fish species inside and outside the Mekong Delta.\",\"PeriodicalId\":36378,\"journal\":{\"name\":\"Veterinary Integrative Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Integrative Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12982/vis.2022.049\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Veterinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Integrative Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12982/vis.2022.049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Veterinary","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在提供一种对鱼类性腺进行显微观察的方法。本研究中使用的标本是2022年4月至7月在湄公河三角洲收集的鱼类的卵巢和睾丸。两性各有五个不同阶段的器官(卵巢直径从第一阶段的1.16厘米到第五阶段的4.04厘米;睾丸直径从I期的0.42到V期的2.39)。结果表明,卵巢和睾丸均需在4%福尔摩溶液中固定24 h,并在流水下固定8小时。然后将卵巢和睾丸浸泡在500乙醇中24 h以开始脱水。结果表明,卵巢脱水和石蜡浸润时间(26 ~ 55 h)大于睾丸(25 ~ 26 h)。苏木精对卵巢的平均染色时间为1.5 min (I期)~ 3.5 min (V期),伊红对卵巢的平均染色时间为50 s (I期)~ 140 s (V期)。而睾丸染色时间较短,苏木精染色时间为1 min (I期)~ 3 min (V期),伊红染色时间为30 s (I期)~ 90 s (V期)。本研究为进一步研究鱼类性腺的组织学结构提供了参考。这些结果可以应用于湄公河三角洲内外的鱼类。
Procedure for performing a fixed microscopic specimen of the gonads of fish
This study aims to provide a procedure for performing microscopy in fish gonads. Specimens used in this study were the ovaries and testes of fish collected in the Mekong Delta from April to July 2022. Organs at five different stages for each sex (ovary diameter from 1.16 cm in stage I to 4.04 cm in stage V; testis diameter from 0.42 in stage I to 2.39 in stage V) were studied in this procedure. The results showed that both ovaries and testes needed to be fixed at 4% formol for 24 h and under running water for eight hours. Ovaries and testes were then immersed in 500 ethanol for 24 h to initiate dehydration. Results showed that the time for dehydration and paraffin infiltration was longer in the ovary (26 to 55 h across stages) than in the testis (25 to 26 h across stages). The mean staining time of the ovary was 1.5 min (stage I) to 3.5 min (stage V) in Hematoxylin and 50 s (stage I) to 140 s (stage V) in Eosin. Whereas the staining time of testis was shorter, 1 min (stage I) to 3 min (stage V) in Hematoxylin and 30 s (stage I) to 90 s (stage V) in Eosin. This study provides a reference for further studies on the histological structure of fish gonads. These results could be applied to fish species inside and outside the Mekong Delta.