{"title":"利用天然荧光评估珊瑚健康状况的高光谱成像工具","authors":"J. Teague, J. Willans, M. Allen, T. Scott, J. Day","doi":"10.1255/JSI.2019.A7","DOIUrl":null,"url":null,"abstract":"Fluorescent proteins are a crucial visualisation tool in a myriad of research fields including cell biology,\nmicrobiology and medicine. Fluorescence is a result of the absorption of electromagnetic radiation at one wavelength and\nits reemission at a longer wavelength. Coral communities exhibit a natural fluorescence which can be used to distinguish\nbetween diseased and healthy specimens, however, current methods, such as the underwater visual census, are\nexpensive and time-consuming constituting many manned dive hours. We propose the use of a remotely operated vehicle\nmounted with a novel hyperspectral fluorescence imaging (HyFI) “payload” for more rapid surveying and data collection.\nWe have tested our system in a laboratory environment on common coral species including Seriatopora spp., Montipora\nverrucosa, Montipora spp., Montipora capricornis, Echinopora lamellose, Euphyllia ancora, Pocillopora damicornis and\nMontipora confusa. With the aid of hyperspectral imaging, the coral specimens’ emission wavelengths can be accurately\nassessed by capturing the emission spectra of the corals when excited with light emitting diodes (395–405 and 440 nm).\nFluorescence can also provide an indicator of coral bleaching as shown in our bleaching experiment where we observe\nfluorescence reduction alongside coral bleaching.","PeriodicalId":37385,"journal":{"name":"Journal of Spectral Imaging","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"10","resultStr":"{\"title\":\"Hyperspectral imaging as a tool for assessing coral health utilising natural fluorescence\",\"authors\":\"J. Teague, J. Willans, M. Allen, T. Scott, J. Day\",\"doi\":\"10.1255/JSI.2019.A7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Fluorescent proteins are a crucial visualisation tool in a myriad of research fields including cell biology,\\nmicrobiology and medicine. Fluorescence is a result of the absorption of electromagnetic radiation at one wavelength and\\nits reemission at a longer wavelength. Coral communities exhibit a natural fluorescence which can be used to distinguish\\nbetween diseased and healthy specimens, however, current methods, such as the underwater visual census, are\\nexpensive and time-consuming constituting many manned dive hours. We propose the use of a remotely operated vehicle\\nmounted with a novel hyperspectral fluorescence imaging (HyFI) “payload” for more rapid surveying and data collection.\\nWe have tested our system in a laboratory environment on common coral species including Seriatopora spp., Montipora\\nverrucosa, Montipora spp., Montipora capricornis, Echinopora lamellose, Euphyllia ancora, Pocillopora damicornis and\\nMontipora confusa. With the aid of hyperspectral imaging, the coral specimens’ emission wavelengths can be accurately\\nassessed by capturing the emission spectra of the corals when excited with light emitting diodes (395–405 and 440 nm).\\nFluorescence can also provide an indicator of coral bleaching as shown in our bleaching experiment where we observe\\nfluorescence reduction alongside coral bleaching.\",\"PeriodicalId\":37385,\"journal\":{\"name\":\"Journal of Spectral Imaging\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Spectral Imaging\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1255/JSI.2019.A7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Chemistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Spectral Imaging","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1255/JSI.2019.A7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Chemistry","Score":null,"Total":0}
Hyperspectral imaging as a tool for assessing coral health utilising natural fluorescence
Fluorescent proteins are a crucial visualisation tool in a myriad of research fields including cell biology,
microbiology and medicine. Fluorescence is a result of the absorption of electromagnetic radiation at one wavelength and
its reemission at a longer wavelength. Coral communities exhibit a natural fluorescence which can be used to distinguish
between diseased and healthy specimens, however, current methods, such as the underwater visual census, are
expensive and time-consuming constituting many manned dive hours. We propose the use of a remotely operated vehicle
mounted with a novel hyperspectral fluorescence imaging (HyFI) “payload” for more rapid surveying and data collection.
We have tested our system in a laboratory environment on common coral species including Seriatopora spp., Montipora
verrucosa, Montipora spp., Montipora capricornis, Echinopora lamellose, Euphyllia ancora, Pocillopora damicornis and
Montipora confusa. With the aid of hyperspectral imaging, the coral specimens’ emission wavelengths can be accurately
assessed by capturing the emission spectra of the corals when excited with light emitting diodes (395–405 and 440 nm).
Fluorescence can also provide an indicator of coral bleaching as shown in our bleaching experiment where we observe
fluorescence reduction alongside coral bleaching.
期刊介绍:
JSI—Journal of Spectral Imaging is the first journal to bring together current research from the diverse research areas of spectral, hyperspectral and chemical imaging as well as related areas such as remote sensing, chemometrics, data mining and data handling for spectral image data. We believe all those working in Spectral Imaging can benefit from the knowledge of others even in widely different fields. We welcome original research papers, letters, review articles, tutorial papers, short communications and technical notes.