利用天然荧光评估珊瑚健康状况的高光谱成像工具

Q3 Chemistry
J. Teague, J. Willans, M. Allen, T. Scott, J. Day
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引用次数: 10

摘要

荧光蛋白是细胞生物学、微生物学和医学等众多研究领域的重要可视化工具。荧光是吸收一个波长的电磁辐射,然后在更长的波长重新发射的结果。珊瑚群落表现出天然荧光,可用于区分患病标本和健康标本,然而,目前的方法,如水下视觉普查,既昂贵又耗时,需要许多人工潜水时间。我们建议使用装有新型高光谱荧光成像(HyFI)“有效载荷”的远程操作车辆进行更快速的测量和数据收集。我们在实验室环境中对常见珊瑚物种进行了系统测试,包括Seriatora spp.、Montiporaverucosa、Montipora spp.、Monteipora capricornis、Echinopora lamellose、Euphillia ancora、Pocillopora damicornis和Montipora confusa。借助高光谱成像,当用发光二极管(395–405)激发时,可以通过捕捉珊瑚的发射光谱来准确评估珊瑚标本的发射波长 和 440nm)。荧光也可以提供珊瑚漂白的指示剂,如我们的漂白实验所示,在该实验中,我们观察到珊瑚漂白的同时荧光减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hyperspectral imaging as a tool for assessing coral health utilising natural fluorescence
Fluorescent proteins are a crucial visualisation tool in a myriad of research fields including cell biology, microbiology and medicine. Fluorescence is a result of the absorption of electromagnetic radiation at one wavelength and its reemission at a longer wavelength. Coral communities exhibit a natural fluorescence which can be used to distinguish between diseased and healthy specimens, however, current methods, such as the underwater visual census, are expensive and time-consuming constituting many manned dive hours. We propose the use of a remotely operated vehicle mounted with a novel hyperspectral fluorescence imaging (HyFI) “payload” for more rapid surveying and data collection. We have tested our system in a laboratory environment on common coral species including Seriatopora spp., Montipora verrucosa, Montipora spp., Montipora capricornis, Echinopora lamellose, Euphyllia ancora, Pocillopora damicornis and Montipora confusa. With the aid of hyperspectral imaging, the coral specimens’ emission wavelengths can be accurately assessed by capturing the emission spectra of the corals when excited with light emitting diodes (395–405 and 440 nm). Fluorescence can also provide an indicator of coral bleaching as shown in our bleaching experiment where we observe fluorescence reduction alongside coral bleaching.
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来源期刊
Journal of Spectral Imaging
Journal of Spectral Imaging Chemistry-Analytical Chemistry
CiteScore
3.90
自引率
0.00%
发文量
11
审稿时长
22 weeks
期刊介绍: JSI—Journal of Spectral Imaging is the first journal to bring together current research from the diverse research areas of spectral, hyperspectral and chemical imaging as well as related areas such as remote sensing, chemometrics, data mining and data handling for spectral image data. We believe all those working in Spectral Imaging can benefit from the knowledge of others even in widely different fields. We welcome original research papers, letters, review articles, tutorial papers, short communications and technical notes.
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