富含阿尔及利亚椰枣花粉的扩张剂对不同年龄兔精液冷冻特性的影响

IF 0.8 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
A. Laghouati, R. Belabbas, S. Mattioli, A. Dal Bosco, A. Benberkane, Elisabetta Bravi, V. Sileoni, O. Marconi, C. Castellini
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引用次数: 0

摘要

在本研究中,我们评估了不同浓度的椰枣花粉(DPP)水提取物对兔精子在4°C下储存48小时的影响。使用人工阴道收集了老年和年轻兔雄鹿的精液,并初步评估了精子质量。精子用添加(20、40、80 mg/mL) DPP水提物的Tris缓冲液稀释。延长后的样品在4°C下保存48小时。在保存2、4、24和48小时后,评估精子活力和运动动力学。每次冷冻一份等分液,用于分析评价硫代巴比妥酸反应物质(TBARS)(脂质过氧化)和工具(维生素E)。评估DPP中的近似组成、抗氧化剂和糖含量。结果表明,DPP具有高蛋白(27.10%)和灰分(18.43%)的特点,而脂肪含量很低(0.51%),总糖含量也很高(16.25 g/100 g新鲜物质,f.m)。抗氧化剂总量为26.48 mg/g f.m,总多酚含量为5.01 mg没食子酸当量/g f.m,多不饱和脂肪酸含量在30%左右。枣椰树花粉提取物对老龄家兔精子参数(主要是曲线速度[VCL])有剂量依赖性影响,但在剂量>DPP40时无影响或负影响。相反,在最佳条件下(即幼兔精液),添加花粉对精子性状(活力和VCL)没有影响。DPP的逐渐增加,尽管为精子提供了额外数量的工具,但也导致了更高的工具消耗和脂质氧化的增加。特别是,DPP80增加了两个年龄兔精子中的TBARS水平。在DPP20中发现了更好的趋势,在老年和年轻雄鹿中,DPP20的工具消耗率分别为10.46%和15.28%,与高剂量DPP相比,脂质氧化较低。综上所述,本研究结果表明,在Tris缓冲剂中添加DPP提取物,只有在老兔精液中添加浓度低于40 mg/mL时,才能提高冷冻兔精液的性状。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of an extender enriched with algerian date palm pollen on chilled semen characteristics of rabbit bucks at different ages
In the present study, we evaluated the effect of various concentrations of date palm pollen (DPP) aqueous extract on rabbit sperm during storage at 4°C for 48 h. Semen was collected from old and young rabbit bucks using an artificial vagina and initially evaluated for sperm quality. The sperm were diluted in Tris buffer supplemented with (20, 40, 80 mg/mL) of DPP aqueous extract. The extended samples were stored at 4°C for 48 h. Sperm motility and motion kinetics were assessed after 2, 4, 24 and 48 h of storage. At each time, an aliquot was frozen for the analytical evaluation of thiobarbituric acid reactive substances (TBARS) (lipid peroxidation) and tocols (vitamin E). Proximate composition, antioxidant and sugar content were evaluated in DPP. The results showed that DPP was characterised by a high proportion of protein (27.10%) and ash (18.43%), whereas the lipid fraction was very low (0.51%) and total sugar was also high (16.25 g/100 g of fresh matter, f.m.). Regarding the antioxidants content, the sum of tocols showed a total value of 26.48 mg/g f.m. The total polyphenols content was 5.01 mg gallic acid equivalents/g f.m, and polyunsaturated fatty acids was around 30%. Date palm pollen extract had a dose-dependent effect on sperm parameters (curvilinear velocity [VCL] mainly) of old rabbit, although a null or negative effect was recorded at doses >DPP40. Conversely, in optimal conditions (i.e. young rabbit semen), the addition of pollen had no effect on sperm traits (motility and VCL). The progressive increase in DPP, despite providing sperm with an additional amount of tocols, also caused higher tocol consumption and an increase in lipid oxidation. In particular, DPP80 increased the TBARS level in sperm of both rabbit ages. The better trend was found in DPP20, where the tocol consumption figures were 10.46 % and 15.28 %, respectively, in old and young bucks, and the lipid oxidation was lower compared to the higher doses of DPP. In conclusion, the findings of this study demonstrated that supplementation of DPP extract to Tris buffer extender enhanced chilled rabbit semen traits only if administered in old rabbit semen at concentration lower than 40 mg/mL.
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来源期刊
World Rabbit Science
World Rabbit Science 农林科学-奶制品与动物科学
CiteScore
1.70
自引率
25.00%
发文量
22
审稿时长
>36 weeks
期刊介绍: World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.
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