P. Apridamayanti, Hadi Kurniawan, F. Nugraha, Inarah Fajriaty, S. Nurbaeti, L. Pratiwi
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The Total phenolic content was determined by the Follin ciocalticeu method with UV/Vis spectrophotometry. Furthermore, antioxidant activity tests with DPPH and FRAP methods using UV/Vis spectrophotometry and ELISA to determine antioxidant activity. The mineral content was observed using the molybdate vandalate method with UV/vis spectrophotometry and ICP-MS, Heavy metal contamination using the ICP-MS tool, and microbial contamination using the plate method. Moreover, the infundation process was carried out on banana and pineapple peels, and the results were evaporated using a food dehydrator to obtain a combination extract. The results showed that the organoleptically KP-KN combination extract had a bitter taste, pineapple smells, 15.57% ash content, ethanol soluble extract content of 55.10%, and 47.99% water-soluble extract. Also, the total phenolic of 6.22 g/Kg, as well as the mineral content of Potassium (K), Calcium (Ca), Iron (Fe), Magnesium (Mg), Zinc (Zn), and Phosphorus (P), which were 47.23 g/Kg, 291.43 mg/Kg, <1x10-6 g/Kg, 2.59x10-3 g/Kg, and 2.38 g/Kg, respectively. Meanwhile, the heavy metal and bacterial contamination test showed that Arsenic (As), Sn, Mercury (Hg), and lead (Pb) were <1x10-6 g/Kg and Cadmium (Cd) was 0.0293. In the microbial contamination test with bacterial components including the ALT test, a value of 4.83 x 103 cfu/g was found, and there was no contamination from E.coli, mold, and yeast bacteria, DPPH and FRAP methods showed antioxidant activity combination extract banana peels and pineapple peels with values of 1390.96±2.83 mg/L and 4542.81±1.10 mg/L compare antioxidant activity of ascorbic acid as standard is 2.13 mg/L and 4.83 mg/L. 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The mineral content was observed using the molybdate vandalate method with UV/vis spectrophotometry and ICP-MS, Heavy metal contamination using the ICP-MS tool, and microbial contamination using the plate method. Moreover, the infundation process was carried out on banana and pineapple peels, and the results were evaporated using a food dehydrator to obtain a combination extract. The results showed that the organoleptically KP-KN combination extract had a bitter taste, pineapple smells, 15.57% ash content, ethanol soluble extract content of 55.10%, and 47.99% water-soluble extract. Also, the total phenolic of 6.22 g/Kg, as well as the mineral content of Potassium (K), Calcium (Ca), Iron (Fe), Magnesium (Mg), Zinc (Zn), and Phosphorus (P), which were 47.23 g/Kg, 291.43 mg/Kg, <1x10-6 g/Kg, 2.59x10-3 g/Kg, and 2.38 g/Kg, respectively. 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引用次数: 0
摘要
Kepok香蕉和菠萝是西加里曼丹的园艺商品。这两种水果通常被用作加工食品,如炸香蕉和菠萝酱。使用水果作为食物通常会留下果皮作为副产品,这会产生两倍于所消耗食物重量的废物。同时,废物利用是将食品加工成功能性产品并发展成硬糖的一项创新。因此,本研究旨在对制作糖果的原料,即香蕉和菠萝皮提取物的组合(KP-KN)进行特定和非特定的表征。表征是对乙醇可溶性和水溶性提取物含量的感官测试。采用Follin ciocalticeu法,紫外/可见分光光度法测定总酚含量。此外,采用DPPH和FRAP法进行抗氧化活性测试,采用紫外/可见分光光度法和ELISA法测定抗氧化活性。使用钼酸盐-钒酸盐法、紫外/可见分光光度法和ICP-MS观察矿物含量,使用ICP-MS工具观察重金属污染,使用平板法观察微生物污染。此外,对香蕉皮和菠萝皮进行了浸渍处理,并使用食品脱水器蒸发结果,以获得组合提取物。结果表明,KP-KN组合提取物具有苦味、菠萝味、15.57%的灰分、55.10%的乙醇溶性提取物和47.99%的水溶性提取物。此外,总酚含量为6.22g/Kg,钾(K)、钙(Ca)、铁(Fe)、镁(Mg)、锌(Zn)和磷(P)的矿物含量分别为47.23g/Kg、291.43mg/Kg、<1x10-6g/Kg、2.59x10-3g/Kg和2.38g/Kg。同时,重金属和细菌污染试验表明,砷(As)、锡(Sn)、汞(Hg)和铅(Pb)<1x10-6 g/Kg,镉(Cd)为0.0293。在包括ALT测试在内的细菌成分的微生物污染测试中,发现值为4.83 x 103 cfu/g,没有来自大肠杆菌、霉菌和酵母的污染,DPPH法和FRAP法测定香蕉皮和菠萝皮提取物的抗氧化活性组合值分别为1390.96±2.83mg/L和4542.81±1.10mg/L,抗坏血酸标准品的抗氧化活性分别为2.13mg/L和4.83mg/L。KP-KN组合提取物的测试可以作为制作硬糖的原料,因为它含有身体所需的矿物质,符合传统药物的安全和质量要求,并且在体外具有抗氧化活性。
Characterization and Antioxidant Activity of Banana Peels, Pineapple Peels, and Combination Extracts of both Peels as Raw Materials in the Development of Hard Candy
Kepok banana and pineapple are horticultural commodities found in West Kalimantan. The two fruits are generally used as processed foods such as fried bananas and pineapple jam. The use of fruit as food often leaves the peel as a by-product, which can produce waste twice the weight of the food consumed. Meanwhile, waste utilization is an innovation in food processing into functional products which are developed into hard candy. Therefore, this research aims to the specific and non-specific characterization of raw materials for making candy, namely the combination of banana and pineapple peel extract (KP-KN). The characterization is organoleptic tests on the content of ethanol-soluble and water-soluble extracts. The Total phenolic content was determined by the Follin ciocalticeu method with UV/Vis spectrophotometry. Furthermore, antioxidant activity tests with DPPH and FRAP methods using UV/Vis spectrophotometry and ELISA to determine antioxidant activity. The mineral content was observed using the molybdate vandalate method with UV/vis spectrophotometry and ICP-MS, Heavy metal contamination using the ICP-MS tool, and microbial contamination using the plate method. Moreover, the infundation process was carried out on banana and pineapple peels, and the results were evaporated using a food dehydrator to obtain a combination extract. The results showed that the organoleptically KP-KN combination extract had a bitter taste, pineapple smells, 15.57% ash content, ethanol soluble extract content of 55.10%, and 47.99% water-soluble extract. Also, the total phenolic of 6.22 g/Kg, as well as the mineral content of Potassium (K), Calcium (Ca), Iron (Fe), Magnesium (Mg), Zinc (Zn), and Phosphorus (P), which were 47.23 g/Kg, 291.43 mg/Kg, <1x10-6 g/Kg, 2.59x10-3 g/Kg, and 2.38 g/Kg, respectively. Meanwhile, the heavy metal and bacterial contamination test showed that Arsenic (As), Sn, Mercury (Hg), and lead (Pb) were <1x10-6 g/Kg and Cadmium (Cd) was 0.0293. In the microbial contamination test with bacterial components including the ALT test, a value of 4.83 x 103 cfu/g was found, and there was no contamination from E.coli, mold, and yeast bacteria, DPPH and FRAP methods showed antioxidant activity combination extract banana peels and pineapple peels with values of 1390.96±2.83 mg/L and 4542.81±1.10 mg/L compare antioxidant activity of ascorbic acid as standard is 2.13 mg/L and 4.83 mg/L. The test with the KP-KN combined extract can be used as a raw material for making hard candy because it contains minerals needed by the body, meets the safety and quality requirements of traditional medicines, and has antioxidant activity in vitro.