Thore Koppetsch, M. Pabijan, C. Hutter, J. Köhler, Philip-Sebastian Gehring, Andolalao Rakotoarison, F. Ratsoavina, Mark D. Scherz, D. Vieites, F. Glaw, M. Vences
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In-depth study based on DNA sequences of the mitochondrial cytochrome b gene from 338 specimens of G. liber sensu lato from across its range, sequences of four nuclear-encoded markers for 154‒257 of these specimens, a phylogenomic dataset obtained by the FrogCap target capture approach, and additional mitochondrial genes for representatives of most mitochondrial lineages, as well as bioacoustic and morphological comparisons, revealed concordant differentiation among several lineages of the G. liber complex. We identify nine lineages differing by 5.3‒15.5% in cytochrome b and 2.4‒10.1% in the 16S rRNA gene, and find that several of these lack or have only limited allele sharing in the nuclear-encoded genes. Based on sympatric or parapatric occurrence without genetic admixture, combined with differences in bioacoustic and morphological characters, we scientifically name three lineages from northern Madagascar as new species: G. razokysp. nov., G. razandrysp. nov., and G. fotsitendasp. nov. Of these new species, G. razokysp. nov. and G. razandrysp. nov. show widespread syntopy across northern Madagascar and differ in body size and advertisement calls. Guibemantis fotsitendasp. nov. is sister to G. razandrysp. nov., but appears to occur at lower elevations, including in close geographic proximity on the Marojejy Massif. We also detected subtle differences in advertisement calls among various other mitochondrial lineages distributed in the Northern Central East and Southern Central East of Madagascar, but the status and nomenclatural identity of these lineages require further morphological and bioacoustic study of reliably genotyped individuals, and assignment of the three available names in the complex: Rhacophorus liber Peracca, 1893, Gephyromantis albogularis Guibé, 1947, and Gephyromantis variabilis Millot and Guibé, 1951. We discuss the identity and type material of these three nomina, designate a lectotype for Gephyromantis variabilis from Itremo, and flag the collection of new material from their type localities, Andrangoloaka and Itremo, as paramount for a comprehensive revision of the G. liber complex.","PeriodicalId":51290,"journal":{"name":"Vertebrate Zoology","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar\",\"authors\":\"Thore Koppetsch, M. Pabijan, C. Hutter, J. Köhler, Philip-Sebastian Gehring, Andolalao Rakotoarison, F. Ratsoavina, Mark D. Scherz, D. Vieites, F. Glaw, M. 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In-depth study based on DNA sequences of the mitochondrial cytochrome b gene from 338 specimens of G. liber sensu lato from across its range, sequences of four nuclear-encoded markers for 154‒257 of these specimens, a phylogenomic dataset obtained by the FrogCap target capture approach, and additional mitochondrial genes for representatives of most mitochondrial lineages, as well as bioacoustic and morphological comparisons, revealed concordant differentiation among several lineages of the G. liber complex. We identify nine lineages differing by 5.3‒15.5% in cytochrome b and 2.4‒10.1% in the 16S rRNA gene, and find that several of these lack or have only limited allele sharing in the nuclear-encoded genes. Based on sympatric or parapatric occurrence without genetic admixture, combined with differences in bioacoustic and morphological characters, we scientifically name three lineages from northern Madagascar as new species: G. razokysp. nov., G. razandrysp. nov., and G. fotsitendasp. nov. Of these new species, G. razokysp. nov. and G. razandrysp. nov. show widespread syntopy across northern Madagascar and differ in body size and advertisement calls. Guibemantis fotsitendasp. nov. is sister to G. razandrysp. nov., but appears to occur at lower elevations, including in close geographic proximity on the Marojejy Massif. 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引用次数: 0
摘要
小型树栖蛙Guibemantis liber(无尾目:Mantellidae)是存在深层同源谱系的一个例子,这些谱系在线粒体DNA上存在大量差异,但在形态和生物声学上相似,因此被分配到相同的名义物种。在马达加斯加北部的田野调查中,我们发现了更多这样的谱系,令人惊讶的是,在不同的地点观察到其中两个谱系的相似特征。深入研究了来自其分布范围内的338个G. liber sensu lato标本的线粒体细胞色素b基因DNA序列,其中154-257个标本的四个核编码标记序列,通过FrogCap靶捕获方法获得的系统基因组数据集,以及大多数线粒体谱系代表的额外线粒体基因,以及生物声学和形态学比较。揭示了G. liber复合体的几个谱系之间的一致分化。我们鉴定了9个细胞色素b和16S rRNA基因差异分别为5.3-15.5%和2.4-10.1%的世系,发现其中一些在核编码基因中缺乏或只有有限的等位基因共享。基于无遗传混杂的同域或异域发生,结合生物声学和形态特征的差异,我们将马达加斯加北部的三个谱系科学命名为新种:G. razokysp。11月,G. razandrysp。和G. fotsitendasp。11 .在这些新种中,11月和G. razandrysp。11 .在马达加斯加北部广泛分布,体型和叫声不同。Guibemantis fotsitendasp。11月是G. razandrysp的妹妹。11月,但似乎发生在较低的海拔,包括在地理上接近Marojejy地块。我们还在分布在马达加斯加中东部北部和中东部南部的各种线粒体谱系中发现了广告呼叫的细微差异,但这些谱系的地位和命名身份需要对可靠的基因分型个体进行进一步的形态学和生物声学研究,并在复体中分配三个可用名称:Peracca, 1893; Gephyromantis albogularis guib, 1947; Gephyromantis variabilis Millot and guib, 1951。我们讨论了这三种候选植物的身份和类型材料,为来自Itremo的Gephyromantis variabilis指定了一种类型,并标记了来自其类型所在地Andrangoloaka和Itremo的新材料的收集,作为G. liber复合物全面修订的重要内容。
An initial molecular resolution of the mantellid frogs of the Guibemantis liber complex reveals three new species from northern Madagascar
The small arboreal frog Guibemantis liber (Anura: Mantellidae) has served as an example for the existence of deep conspecific lineages that differ by a substantial amount in mitochondrial DNA but are similar in morphology and bioacoustics and thus are assigned to the same nominal species. During fieldwork in northern Madagascar, we identified additional such lineages and surprisingly, observed close syntopy of two of these at various sites. In-depth study based on DNA sequences of the mitochondrial cytochrome b gene from 338 specimens of G. liber sensu lato from across its range, sequences of four nuclear-encoded markers for 154‒257 of these specimens, a phylogenomic dataset obtained by the FrogCap target capture approach, and additional mitochondrial genes for representatives of most mitochondrial lineages, as well as bioacoustic and morphological comparisons, revealed concordant differentiation among several lineages of the G. liber complex. We identify nine lineages differing by 5.3‒15.5% in cytochrome b and 2.4‒10.1% in the 16S rRNA gene, and find that several of these lack or have only limited allele sharing in the nuclear-encoded genes. Based on sympatric or parapatric occurrence without genetic admixture, combined with differences in bioacoustic and morphological characters, we scientifically name three lineages from northern Madagascar as new species: G. razokysp. nov., G. razandrysp. nov., and G. fotsitendasp. nov. Of these new species, G. razokysp. nov. and G. razandrysp. nov. show widespread syntopy across northern Madagascar and differ in body size and advertisement calls. Guibemantis fotsitendasp. nov. is sister to G. razandrysp. nov., but appears to occur at lower elevations, including in close geographic proximity on the Marojejy Massif. We also detected subtle differences in advertisement calls among various other mitochondrial lineages distributed in the Northern Central East and Southern Central East of Madagascar, but the status and nomenclatural identity of these lineages require further morphological and bioacoustic study of reliably genotyped individuals, and assignment of the three available names in the complex: Rhacophorus liber Peracca, 1893, Gephyromantis albogularis Guibé, 1947, and Gephyromantis variabilis Millot and Guibé, 1951. We discuss the identity and type material of these three nomina, designate a lectotype for Gephyromantis variabilis from Itremo, and flag the collection of new material from their type localities, Andrangoloaka and Itremo, as paramount for a comprehensive revision of the G. liber complex.
期刊介绍:
Research fields covered by VERTEBRATE ZOOLOGY are taxonomy, morphology, anatomy, phylogeny (molecular and morphology-based), historical biogeography, and palaeontology of vertebrates.