TiO2纳米粒子对光催化过程中应用最广泛的胶质母细胞瘤和神经母细胞瘤的毒性研究

IF 1.3 Q4 ENVIRONMENTAL SCIENCES
F. Kazemi, Marzie Esmaeeli, Peyman Mohammadzadehjahani, Mahnaz Amiri, Parisa Vosough, Meysam Ahmadi-Zeidabadi
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引用次数: 1

摘要

背景:二氧化钛(TiO2)纳米颗粒(NP)是最重要和最有用的光催化剂之一。最近,人们已经考虑了这些NP的生物学特性,特别是其抗癌活性。胶质母细胞瘤和神经母细胞瘤是人类死亡率较高的两种致命脑肿瘤,常用方法治疗的希望渺茫。方法:本研究观察了TiO2单独和联合紫外线照射对神经母细胞瘤(SH-SY5Y)和胶质母细胞瘤U87两种不同细胞系的细胞毒性作用。在给予10、50、100和500μg/mL TiO2、0.043和1.4mW/cm2 UVA照射后,通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物(MTT)测定在4、24和48小时后研究细胞活力。结果:MTT法和光学显微镜显示,TiO2纳米粒子的作用因物质剂量、冲击时间、细胞类型和辐射量而异。在这项研究中,单独使用NP,观察到该物质的毒性和无毒性。对于UV存在下的NP,基于其单独状态的比较以及两组的活力测定的差异,观察到了NP的光催化和涂层效果。结论:根据结果,不同浓度的TiO2可以用于不同的目的。低浓度的TiO2可用于提高光动力治疗的效率,而高浓度的TiO2则可用于保护正常细胞。这种策略改进了光动力疗法并减少了有害影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of toxicity of TiO2 nanoparticles on glioblastoma and neuroblastoma as the most widely used nanoparticles in photocatalytic processes
Background: Titanium dioxide (TiO2 ) nanoparticles (NPs) are among the most important and usable photocatalysts. Recently, the biological properties of these NPs, particularly, its anticancer activity, have been considered. Glioblastoma and neuroblastoma are two fatal brain tumors with a high mortality rate in humans, the hope for treatment of which is weak by the common methods. Methods: In this study, the cytotoxicity effects of TiO2 alone and in combination with ultraviolet A (UVA) irradiation on two different cell lines, neuroblastoma (SH-SY5Y) and glioblastoma U87, were investigated. After administration of 10, 50, 100, and 500 μg/mL TiO2 , 0.043 and 1.4 mW/cm2 UVA irradiation, cell viability was investigated after 4, 24, and 48 hours by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Results: MTT assay and light microscope demonstrated that the effect of TiO2 NPs varied based on the dose of the substance, the impact time, the cell type, and the amount of radiation. In this study, for NPs alone, both toxicity and non-toxicity of the substance were observed. For NPs in the presence of UV, based on the comparison with its status alone and the difference in the viability assay of the two groups, both the photocatalytic and the coating effect of the NPs were observed. Conclusion: According to the results, different concentrations of TiO2 can be used for different purposes. Low concentrations of TiO2 can be used to increase the efficiency of photodynamic therapy and high concentrations of TiO2 can be used to protect the normal cell. This strategy improves the photodynamic therapy and reduces the harmful effects.
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来源期刊
CiteScore
2.40
自引率
37.50%
发文量
17
审稿时长
12 weeks
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