新南威尔士州地区育龄妇女尿路致病性大肠杆菌的抗生素敏感性模式和生物膜的产生

Kudinha Timothy, Kong Fanrong
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引用次数: 0

摘要

背景:尿路感染(UTIs)是一种重要的人类感染,主要由尿路致病性大肠杆菌(UPEC)引起。了解UTI的发病机制对于对抗治疗失败和复发性感染的增加非常重要。我们研究了来自育龄妇女的UPEC抗生素耐药性和敏感性膀胱炎分离株中宿主和非宿主因子产生生物膜的分布。方法:测试来自女性的膀胱炎分离株(n=534)(i)使用微量滴定板法生产生物膜,(ii)使用CLSI圆盘法对14种抗生素的易感性,最后,(iii)使用改进的Clermont法的系统发育群状态。结果:共对534株女性UPEC分离株进行了研究。26-30岁年龄组的生物膜产量最高(73%),16-20岁年龄组最低(35%)。来自住院患者的分离株(72%)产生生物膜的比例更大,而门诊患者的比例为45%。大多数复发性尿路感染分离株(92%)是生物膜产生者,而首次感染的比例为48%。大多数分离株(61%)对一种或多种抗生素具有耐药性。在绝大多数情况下,耐药分离株与易感分离株的生物膜产量最高,平均是易感分离菌的两倍。与任何其他组相比,B2分离株中有更高比例(77%)是生物膜生产者。结论:我们的研究结果表明,生物膜的产生与几种宿主和非宿主因素密切相关,包括年龄、尿路感染复发、住院治疗以及系统发育组B2和D。这些因素与多药耐药性有关,表明生物膜的生产与多药耐药之间有着密切的联系,如前所述。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibiotic Susceptibility Patterns and Biofilm Production by Uropathogenic Escherichia coli from Reproductive Age Women in a Region of NSW
Background: Urinary tract infections (UTIs), mostly caused by uropathogenic E. coli (UPEC), are important human infections. Understanding UTI pathogenesis is important for combating the rise in treatment failure and recurrent infections. We studied the distribution of biofilm production by host and non-host factors, among UPEC antibiotic resistant and sensitive cystitis isolates, from reproductive age women. Methods: Cystitis isolates (n = 534) from women were tested for (i) Biofilm production using a microtitre plate method, (ii) Susceptibility to 14 antibiotics using the CLSI disk method, and finally, (iii) Phylogenetic group status using the improved Clermont method. Results: A total of 534 UPEC isolates from women were studied. Biofilm production was highest in the 26-30 years age group (73%), and lowest in the 16-20 years age group (35%). A greater proportion of isolates from inpatients (72%) produced biofilms vs. 45% for outpatients. Most of recurrent UTI isolates (92%) were biofilm producers vs. 48% for first time UTI. The majority of the isolates (61%) were resistant to one or more antibiotics. In the overwhelming majority of cases, biofilm production was highest among the resistant isolates vs. susceptible ones, on average by twice as much. A higher proportion of B2 isolates (77%) were biofilm producers compared to any another group. Conclusion: Our findings suggest a strong association between biofilm production and several host and non-host factors, including age, UTI recurrence, hospitalisation, and phylogenetic groups B2 and D. These factors are associated with multidrug resistance, suggesting a strong link between biofilm production and multidrug resistance, as previously suggested.
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