赛鸽α -珠蛋白基因多态性与其表达水平的关系

Magdalena Jedrzejczak-Silicka, Krystyna Dudaniec, A. Dybus
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引用次数: 1

摘要

本研究旨在探讨鸽子α - a -珠蛋白(AGLOB)基因多态性对其表达水平的影响。分析了位于该基因3′侧区的g.5768C>T取代。采用PCR-RFLP (AvaII)方法进行SNP鉴定。采用qPCR进行基因表达分析。结果表明,不同g.5768C>T基因型鸽子α - a -珠蛋白基因表达水平差异不显著。AGLOBCC基因型与鸽子α - a -珠蛋白基因的表达量比AGLOBTT基因型高2.46倍。α a -珠蛋白基因在AGLOBTT鸽子中的低表达可能与所分析基因区域中存在顺式调控元件有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association of alpha-a globin gene polymorphism with its expression level in racing pigeons
The objective of the study was to investigate possible effect of the polymorphism in pigeon αA-globin (AGLOB) gene on its expression level. The g.5768C>T substitution located in 3′ flanking region of the gene was analyzed. PCR-RFLP (AvaII) method was used to identify the SNP. The gene expression analyses were performed using qPCR. The investigation indicated a difference but not significant in the αA-globin gene expression levels between pigeons carrying different g.5768C>T genotypes. The AGLOBCC genotype was associated with 2.46-fold higher expression level of pigeon αA-globin gene compared to AGLOBTT genotype. The lower expression of αA-globin gene in AGLOBTT pigeons may be caused by the presence of cis-acting regulatory elements in the analysed gene region.
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