WILD BARBERRY FRUIT (Berberis microphylla G. Forst.) AS A NATURAL INGREDIENT FOR BEER BREWING

Barberry ( Berberis microphylla ) is a wild berry endemic to the Andean-Patagonian zone of Argentina and Chile. Even though it is not widely used or consumed, it has great potential for coloring and enrichment of different food matrices due to its high anthocyanin and antioxidant contents. The objective of this study was to evaluate the impact of barberry addition on quality parameters and stability of sour beer. Nutritional, physicochemical and sensory characteristics of the beer were analyzed. Firstly, different amounts of freeze-dried barberry were added to sour beer (0, 2.5, 5, and 10 g L -1 ) after primary fermentation. In the next experiment, beers brewed with 0 (control) and 5 g L -1 of barberry were stored at 5ºC for up to 90 days and evaluated on a monthly basis. As demonstrated by the CIE Lab and Abs 520nm parameters, barberry addition resulted in a marked color shift towards ruby red tones. It also increased TEAC and phenolic contents by 2-4 times, without affecting beer pH, acidity, or density. Furthermore, it had a positive impact on overall impression, appearance, aroma, flavor and balanced scores in a trained sensorial panel. Beer total anthocyanins varied between 30 and 100 mg D3G L -1 . The beers brewed with barberry fruit presented stable antioxidant capacity, total anthocyanin content and both anthocyanin ionization and polymerization degree during storage. The results show that barberry can be used as an ingredient to make red and antioxidant-enriched sour beers with good stability during storage. ); B: (Abs 520; pH<1 - Abs 700; pH<1 ); DF: dilution factor Anthocyanin degradation products were measured by polymeric color assay. The degassed beer sample was diluted with distilled water. A 2.8 mL-aliquot portion of the dilution was placed in a test tube and 0.2 mL of S (20% freshly prepared)