不同浓度椰子水在18°C下平衡不同时间对猪精液的影响研究

Q3 Veterinary

American Journal of Animal and Veterinary Sciences Pub Date : 2022-04-01 DOI:10.3844/ajavsp.2022.281.287

Mduduzi M. Tshabalala, M. Mphaphathi, T. R. Netshirovha, K. Nephawe, C. Pilane, Lucky Nedambale

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引用次数: 0

摘要

一种丰富的自然资源，如椰子水(CCW)具有高浓度的抗氧化剂、营养物质、氨基酸、糖和电解质。这些草药提取物被用在精液填充剂中以保存动物精子。此外，CCW对体外胚胎的产生也很重要。本研究的目的是研究在18°平衡8、16、24和48小时后，椰子水作为猪精液的替代填充剂的使用情况。使用3头2至3岁的大白×长白猪进行精液收集，采用手套和手技术收集猪精液，收集了24次射精，该研究重复了8次。本研究采用完全随机设计。采用Stata V12统计软件(Stata Corp.， College Station, Tex.)对数据进行方差分析，采用Fisher保护t检验对治疗措施进行分离，显著性值P <0.05。与80%和100% CCW稀释的猪精液相比，60% CCW稀释后平衡8 h的精子活力(86.1±1.7)，活精子率(84.5±20)，质膜(83.3±2.3)和顶体完整性(84.00±2.13)均有所提高。值得注意的是，在80%和100% CCW中稀释并平衡8 h的精液，精子活力(76.2±2.5;63.5±2.7)，活精子(74.5±2.0);63.3±2.1)，完整质膜(73.3±23);63.5±2.0)和平衡后顶体膜完整性(74.0±2.1;63.5±2.7)。然而，用80%和100% CCW稀释的精子在18°C下平衡8、16、24和48小时后，精子活力、活力和血浆和顶体膜的完整性都降低了。综上所述，用60% CCW稀释的平衡猪精液能够在18°C的平衡8、16、24和48℃下保持猪精液参数，如精子活力、活力、质膜和顶体膜完整性。然而，CCW可以作为平衡公猪精液的一种替代添加剂。因此，有必要进一步研究平衡母猪精液在CCW中稀释后的体外和体内受精率。

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Study the Efficacy of Different Concentrations of Coconut Water on Boar Semen Following Equilibration at 18°C for Different Hours

: A rich natural resource such as Coconut Water (CCW) features high concentrations of antioxidants, nutrients, amino acids, sugars, and electrolytes. These herbal extract infusions are used in semen extenders to preserve animal sperm. In addition, CCW is important for in vitro production of embryos. The purpose of this study was to investigate the use of coconut water as an alternative bulking agent for boar semen after equilibration at 18° for 8, 16, 24, and 48 h. Three 2- to 3-year-old Large White x Landrace boars were used for semen collection, boar semen was collected by a glove and hand technique, 24 ejaculates were collected and this study was replicated eight times. A complete randomized design was used in this study. Data were analyzed by ANOVA using Stata V12 statistical software (Stata Corp., College Station, Tex.) and treatment measures were separated using Fisher's protected t-test at significance values of P <0.05. Sperm diluted in 60% CCW and equilibrated for 8 h showed improved sperm motility (86.1±1.7), percentage of viable sperm (84.5±20), plasma membrane (83.3±2.3), and acrosome integrity (84.00±2.13) compared to boar semen diluted with 80 and 100% CCW. Notably, semen diluted in 80 and 100% CCW and equilibrated for 8 h showed improved sperm motility (76.2±2.5; 63.5±2.7), viable sperm (74.5±2.0; 63.3±2.1), intact plasma membrane (73.3±23; 63.5±2.0) and acrosomal membrane integrity after equilibration (74.0±2.1; 63.5±2.7). However, sperm diluted in 80 and 100% CCW had reduced sperm motility, viability, and plasma and acrosomal membrane integrity after 8, 16, 24 and 48 h of equilibration at 18°C. In conclusion, equilibrated boar semen diluted with 60% CCW was able to maintain boar semen parameters such as sperm motility, viability, plasma membrane, and acrosomal membrane integrity following 8, 16, 24, and 48 of equilibration at 18°C. However, CCW can be used as an alternative extender for the equilibration of boar semen. Therefore, further studies are necessary to determine the in vitro and in vivo fertilizing capacity of equilibrium boar semen diluted in CCW.

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来源期刊

American Journal of Animal and Veterinary Sciences Veterinary-Veterinary (all)

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： American Journal of Animal and Veterinary Sciences, a quarterly, peer reviewed publication and is dedicated for publication of research articles in the field of biology of animals and with the scientific understanding of how animals work: from the physiology and biochemistry of tissues and major organ systems down to the structure and function of bio molecules and cells; particular emphasis would given to the studies of growth, reproduction, nutrition and lactation of farm and companion animals and how these processes may be optimized to improve animal re- productivity, health and welfare. Articles in support areas, such as genetics, soils, agricultural economics and marketing, legal aspects and the environment also are encouraged. AJAVS is an important source of researcher to study articles on protection of animal production practices, herd health and monitoring the spread of disease and prevention in both domestic and wild animals.