Poly I:C刺激、Mx转录并促进Labeo catla血液中的抗病毒状态（Hamilton 1822）

IF 0.4 4区农林科学 Q4 FISHERIES

Indian Journal of Fisheries Pub Date : 2021-09-30 DOI:10.21077/ijf.2021.68.3.116333-12

S. Panda, D. Soren, K. K. Nayak, Progyan Roy, B. Das

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引用次数: 1

摘要

本研究对接种poly I: C的Labeo catla (Hamilton 1822)血液中Mx转录本的表达动力学进行了为期14天的研究。与接种磷酸盐缓冲盐水的对照鱼相比，血细胞中Mx转录物的水平增加。定量表达Mx转录物水平，半定量表达β-肌动蛋白。Mx转录物水平在第2天和第3天最高(β-actin: Mx比值约为0.8)，第4天降低，第7天反应消失。血液的免疫印迹显示72 kDa的免疫反应带，这是典型的L. catla Mx蛋白。结果表明，注射poly I: C后，Mx转录物水平升高，可刺激抗病毒阶段，Mx表达研究作为评估保护作用的指标是合适的。关键词:免疫组织化学，黏液病毒抗性，Poly I: C，逆转录酶，Western blotting

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Poly I: C stimulates, Mx transcript and promotes an antiviral state in blood of Labeo catla (Hamilton 1822)

In the current investigation, the kinetics of Mx transcripts expression in the blood of Labeo catla (Hamilton 1822) inoculated with poly I: C, was studied for 14 days. The level of Mx transcript in the blood cells increased as compared to control fish inoculated with phosphate buffered saline. The level of Mx transcript was quantified along with semi-quantitative expression for β-actin. The level of Mx transcript was maximum on day 2 and 3 (β-actin: Mx ratio of about 0.8) which reduced after day 4 and the response disappeared on day 7. Western blots of the blood showed an immune-reactive band of 72 kDa, which is typical for L. catla Mx protein. Results suggest an increase in levels of Mx transcript after poly I: C injection which can stimulate an antiviral phase and the appropriateness of Mx expression investigation as an indicator to assess protective action. Keywords: Immuno-histochemistry, Myxovirus resistance, Poly I: C, Reverse transcriptase, Western blotting

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来源期刊

Indian Journal of Fisheries FISHERIES-

CiteScore

0.90

自引率

20.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Indian Journal of Fisheries is published quarterly by the Indian Council of Agricultural Research (ICAR), New Delhi. Original contributions in the field of Fish and fisheries science are considered for publication in the Journal. The material submitted must be unpublished and not under consideration for publication elsewhere. Papers based on research which kills or damages any species, regarded as thratened/ endangered by IUCN crieteria or is as such listed in the Red Data Book appropriate to the geographic area concerned, will not be accepted by the Journal, unless the work has clear conservation objectives.