刺突酸亚致死浓度协同真菌对麻疯幼虫的致病性（鳞翅目：梨科）

IF 1.3 3区农林科学 Q2 Agricultural and Biological Sciences

European Journal of Entomology Pub Date : 2021-06-01 DOI:10.14411/EJE.2021.015

F. Sohrabi, F. Jamali, J. Michaud

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引用次数: 0

摘要

在实验室条件下，我们评估了四种昆虫病原真菌（EPF）对kuehniella（Zeller）的防治效果及其与生物杀虫剂多杀菌素的兼容性。采用滤纸生物测定法，对三个EPF，包括球孢白僵菌（Balsamo-Criveili）Vuillemin分离株Z1和伊朗1395C，Lecanicillium（=黄萎菌）lecanii（Zimmerman）Zare和Gams，分离株伊朗229，以及Purpureocillium（拟青霉）leculinum（Thom）Luangs-ard，Hywel-Jones和Samson，分离株Iran 1026，进行了抗kuehniella Ephestia三龄和五龄幼虫的试验。三龄和五龄EPF引起的死亡率分别为63.3-72.5%和50-65.5%，LT50分别为8.4-10.5天和10.1-12.9天。对LC10（=26.2ppm）的多杀菌素对EPF孢子萌发的影响进行了评估，发现其可忽略不计，从球孢B.bassiana Z1的0%到紫丁香P.丁香的5.7%。多杀菌素对kuehniella三龄和五龄幼虫的LC50值分别为452.5和1446ppm。随后，在施用LC50的EPFs之前24小时，将LC10的多杀菌素施用于三龄kuehniella幼虫。在L.lecanii、B.bassiana Z1和Iran1395C分离株的应用中添加多杀菌素可协同其对kuehniella幼虫的致病性，而对紫丁香的作用仅为添加剂。我们的结果表明，这些EPF分离株可以与多杀菌素联合有效地用于管理储存产品中的E.kuehniella。

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Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)

We evaluated the efficacy of four entomopathogenic fungi (EPF) and their compatibility with the bioinsecticide spinosad for control of Ephestia kuehniella (Zeller) under laboratory conditions. Three EPF, including Beauveria bassiana (Balsamo-Criveili) Vuillemin isolates Z1 and Iran 1395C, Lecanicillium (= Verticillium) lecanii (Zimmerman) Zare & Gams, isolate Iran 229, and Purpureocillium (Paecilomyces) lilacinum (Thom) Luangs-ard, Hywel-Jones & Samson, isolate Iran 1026 were tested against third and fifth larval instars of Ephestia kuehniella using a filter paper bioassay. Mortality caused by the EPF ranged from 63.3-72.5% for third instars and 50-65.5% for fifth instars, with LT50 ranging from 8.4-10.5 d and 10.1-12.9 d, respectively. The effect of spinosad at LC10 (= 26.2 ppm) on EPF spore germination was evaluated and found to be negligible, ranging from 0% for B. bassiana Z1 to 5.7% for P. lilacinum. The LC50 values for spinosad against third and fifth instar E. kuehniella larvae were 452.5 and 1446 ppm, respectively. Subsequently, spinosad at LC10 was applied to third instar E. kuehniella larvae 24 h before application of the EPFs at LC50. The addition of spinosad to applications of L. lecanii and B. bassiana Z1 and Iran1395C isolates synergized their pathogenicity to E. kuehniella larvae, whereas the effect was merely additive for P. lilacinum. Our results suggest that these EPF isolates can be used effectively in combination with spinosad for management of E. kuehniella in stored products.

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来源期刊

European Journal of Entomology 生物-昆虫学

CiteScore

2.30

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： EJE publishes original articles, reviews and points of view on all aspects of entomology. There are no restrictions on geographic region or taxon (Myriapoda, Chelicerata and terrestrial Crustacea included). Comprehensive studies and comparative/experimental approaches are preferred and the following types of manuscripts will usually be declined: - Descriptive alpha-taxonomic studies unless the paper is markedly comprehensive/revisional taxonomically or regionally, and/or significantly improves our knowledge of comparative morphology, relationships or biogeography of the higher taxon concerned; - Other purely or predominantly descriptive or enumerative papers [such as (ultra)structural and functional details, life tables, host records, distributional records and faunistic surveys, compiled checklists, etc.] unless they are exceptionally comprehensive or concern data or taxa of particular entomological (e.g., phylogenetic) interest; - Papers evaluating the effect of chemicals (including pesticides, plant extracts, attractants or repellents, etc.), irradiation, pathogens, or dealing with other data of predominantly agro-economic impact without general entomological relevance.