脉冲疗法和分子氢在实验性类风湿性关节炎大鼠中的联合应用：疗效的临床和组织学评估

Q3 Pharmacology, Toxicology and Pharmaceutics

Research Results in Pharmacology Pub Date : 2023-05-17 DOI:10.18413/rrpharmacology.9.10024

T. Berezhnova, V. V. Shishkina, D. Esaulenko, Yelena A. Lunyova, Yelena S. Goryushkina, Angelina A. Abramyan, Kseniya S. Dyadina, Anastasia I. Chechelnitsky, Tatiana V. Samoilenko

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引用次数: 0

摘要

简介：糖皮质激素治疗是治疗类风湿性关节炎的基本选择；然而，低剂量和中剂量的长期治疗会产生负面副作用。据报道，氧化应激在类风湿性关节炎的发病机制中至关重要。因此，结合应用甲基强的松龙脉冲治疗和分子氢水溶液的药物治疗似乎是这些病例的合理治疗选择。材料与方法：在成年雄性大鼠身上进行类风湿性关节炎药物联合治疗的疗效研究。通过将牛II型胶原引入右膝关节来模拟类风湿性关节炎。第一组（对照组）的动物接受安慰剂治疗；第二组动物灌胃接受分子富氢水；第3组动物通过尾静脉插管静脉注射甲基强的松龙溶液，第4组动物通过胃内注射富含分子氢的水和静脉注射甲基强的松溶液。动物退出实验后，对其关节组织的显微切片进行组织学分析，并用免疫组织化学方法检测关节炎症的生物标志物。结果和讨论：从第4组动物身上采集的微切片的形态学分析证明了基于炎症生物标志物表达的定量估计的联合治疗的有效性。M1/M2巨噬细胞的动态极化在该组动物中表现出高质量。结论：由于主要的抗氧化物质可以缩短治疗时间，同时确保疾病进程的积极动态，因此对类风湿性关节炎新的治疗选择的搜索范围扩大了。图形摘要：

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Combined use of pulse therapy and molecular hydrogen in rats with experimental rheumatoid arthritis: Clinical and histological evaluation of therapeutic effectiveness

Introduction: Glucocorticosteroid therapy is the basic therapeutic option for rheumatoid arthritis; however, long-term treatment with low and medium doses is associated with the development of negative side effects. As reported, oxidative stress is crucial in the pathogenesis of rheumatoid arthritis. Therefore, pharmacotherapy that combines application of methylprednisolone pulse therapy and an aqueous molecular hydrogen solution seems to be a reasonable treatment option in these cases. Materials and Methods: The study of the effectiveness of combined pharmacotherapy for rheumatoid arthritis was carried out on mature male rats. Rheumatoid arthritis was simulated by introducing bovine type II collagen into the right knee joint. Animals of the 1st – control – group received placebo; animals of the 2nd group received molecular hydrogen-enriched water intragastrically; animals of the 3rd group received methylprednisolone solution intravenously by catheterization of the tail vein, and animals of the 4th group received water enriched with molecular hydrogenintragastrically and methylprednisolone solution intravenously. After withdrawal of animals from the experiment, microsections of their joint tissues were analysed histologically and biomarkers of the joint inflammation were detected immunohistochemically. Results and Discussion: Morphological analysis of microsections taken from animals of the 4th group evidenced effectiveness of the combined therapy based on quantitative estimation of the inflammatory biomarker expression. Dynamic polarization of M1/M2 macrophage was manifested with high quality in animals of this group. Conclusion: The search for new therapeutic options for rheumatoid arthritis is expanded due to major antioxidant substances that can reduce duration of treatment, while ensuring positive dynamics of the course of the disease. Graphical abstract:

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来源期刊

Research Results in Pharmacology Medicine-Pharmacology (medical)

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

12 weeks