{"title":"大规模餐饮场所自来水中微生物质量和军团病风险的长期监测","authors":"Murat Ay","doi":"10.1111/wej.12899","DOIUrl":null,"url":null,"abstract":"Legionnaires' disease is caused by Legionella (L.) pneumophila. Inhaling aerosolized water containing this bacteria is the most common transmission route. Shower heads used in the catering industry's dishwashing units are also potential amplifying systems for bacteria and possible sources of infection by generating droplets and aerosols. Therefore, these systems should not contain pathogenic bacteria. This study aims to monitor the microbial quality, Legionnaires' disease risk and L. pneumophila presence in tap water samples from the water outlet of the shower‐type dishwashing unit of mass catering establishments for a year. Seventy‐two tap water samples were microbiologically tested for total mesophilic aerobic bacteria count, coliform, Escherichia (E.) coli and L. pneumophila. DNA was isolated from L. pneumophila‐positive samples, and their serogroup was characterized by real‐time PCR. The correlation between seasonal variation and prevalence of L. pneumophila serogroup and L. pneumophila and other indicator bacteria were statistically tested by Spearman rank test (p < 0.05). Findings showed that 17 (24%) samples had microbial growth, 14 (19.4%) were positive for L. pneumophila and 13 isolates were characterized as L. pneumophila serogroup 1. The statistical analysis showed no significant correlation between seasonal variation (p = 0.491) and L. pneumophila and total mesophilic aerobic bacteria count, coliform and E. coli (p = 0.923). Overall, Legionella spp. is an essential indicator of the microbial quality in the tap water from mass catering establishments and should be regularly monitored for public health independently from seasonal variation and other indicator bacteria.","PeriodicalId":23753,"journal":{"name":"Water and Environment Journal","volume":" ","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Long‐term monitoring of the microbial quality and Legionnaires' disease risk in tap water from mass catering establishments\",\"authors\":\"Murat Ay\",\"doi\":\"10.1111/wej.12899\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Legionnaires' disease is caused by Legionella (L.) pneumophila. Inhaling aerosolized water containing this bacteria is the most common transmission route. Shower heads used in the catering industry's dishwashing units are also potential amplifying systems for bacteria and possible sources of infection by generating droplets and aerosols. Therefore, these systems should not contain pathogenic bacteria. This study aims to monitor the microbial quality, Legionnaires' disease risk and L. pneumophila presence in tap water samples from the water outlet of the shower‐type dishwashing unit of mass catering establishments for a year. Seventy‐two tap water samples were microbiologically tested for total mesophilic aerobic bacteria count, coliform, Escherichia (E.) coli and L. pneumophila. DNA was isolated from L. pneumophila‐positive samples, and their serogroup was characterized by real‐time PCR. The correlation between seasonal variation and prevalence of L. pneumophila serogroup and L. pneumophila and other indicator bacteria were statistically tested by Spearman rank test (p < 0.05). Findings showed that 17 (24%) samples had microbial growth, 14 (19.4%) were positive for L. pneumophila and 13 isolates were characterized as L. pneumophila serogroup 1. The statistical analysis showed no significant correlation between seasonal variation (p = 0.491) and L. pneumophila and total mesophilic aerobic bacteria count, coliform and E. coli (p = 0.923). Overall, Legionella spp. is an essential indicator of the microbial quality in the tap water from mass catering establishments and should be regularly monitored for public health independently from seasonal variation and other indicator bacteria.\",\"PeriodicalId\":23753,\"journal\":{\"name\":\"Water and Environment Journal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2023-08-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water and Environment Journal\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1111/wej.12899\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water and Environment Journal","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1111/wej.12899","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Long‐term monitoring of the microbial quality and Legionnaires' disease risk in tap water from mass catering establishments
Legionnaires' disease is caused by Legionella (L.) pneumophila. Inhaling aerosolized water containing this bacteria is the most common transmission route. Shower heads used in the catering industry's dishwashing units are also potential amplifying systems for bacteria and possible sources of infection by generating droplets and aerosols. Therefore, these systems should not contain pathogenic bacteria. This study aims to monitor the microbial quality, Legionnaires' disease risk and L. pneumophila presence in tap water samples from the water outlet of the shower‐type dishwashing unit of mass catering establishments for a year. Seventy‐two tap water samples were microbiologically tested for total mesophilic aerobic bacteria count, coliform, Escherichia (E.) coli and L. pneumophila. DNA was isolated from L. pneumophila‐positive samples, and their serogroup was characterized by real‐time PCR. The correlation between seasonal variation and prevalence of L. pneumophila serogroup and L. pneumophila and other indicator bacteria were statistically tested by Spearman rank test (p < 0.05). Findings showed that 17 (24%) samples had microbial growth, 14 (19.4%) were positive for L. pneumophila and 13 isolates were characterized as L. pneumophila serogroup 1. The statistical analysis showed no significant correlation between seasonal variation (p = 0.491) and L. pneumophila and total mesophilic aerobic bacteria count, coliform and E. coli (p = 0.923). Overall, Legionella spp. is an essential indicator of the microbial quality in the tap water from mass catering establishments and should be regularly monitored for public health independently from seasonal variation and other indicator bacteria.
期刊介绍:
Water and Environment Journal is an internationally recognised peer reviewed Journal for the dissemination of innovations and solutions focussed on enhancing water management best practice. Water and Environment Journal is available to over 12,000 institutions with a further 7,000 copies physically distributed to the Chartered Institution of Water and Environmental Management (CIWEM) membership, comprised of environment sector professionals based across the value chain (utilities, consultancy, technology suppliers, regulators, government and NGOs). As such, the journal provides a conduit between academics and practitioners. We therefore particularly encourage contributions focussed at the interface between academia and industry, which deliver industrially impactful applied research underpinned by scientific evidence. We are keen to attract papers on a broad range of subjects including:
-Water and wastewater treatment for agricultural, municipal and industrial applications
-Sludge treatment including processing, storage and management
-Water recycling
-Urban and stormwater management
-Integrated water management strategies
-Water infrastructure and distribution
-Climate change mitigation including management of impacts on agriculture, urban areas and infrastructure